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CMV-LUC2CP/intron/ARE
(Plasmid #62858)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 62858 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL4.16
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4758
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Luciferase
  • Alt name
    firefly luciferase
  • Species
    firefly
  • Insert Size (bp)
    2290
  • Mutation
    insertion of chimeric intron at nueotide poclsition 1344 of the firefly luciferase gene; destabilizing sequences (DS) added to the C terminus of the protein (CL1 and PEST) and five consecutive AUUUA elements added to the 3′ UTR (RNA DS ARE) for rapid mRNA turnover
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-LUC2CP/intron/ARE was a gift from Gideon Dreyfuss (Addgene plasmid # 62858 ; http://n2t.net/addgene:62858 ; RRID:Addgene_62858)
  • For your References section:

    Rapid-response splicing reporter screens identify differential regulators of constitutive and alternative splicing. Younis I, Berg M, Kaida D, Dittmar K, Wang C, Dreyfuss G. Mol Cell Biol. 2010 Apr;30(7):1718-28. doi: 10.1128/MCB.01301-09. Epub 2010 Feb 1. 10.1128/MCB.01301-09 PubMed 20123975