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(Plasmid #62857)


Item Catalog # Description Quantity Price (USD)
Plasmid 62857 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4758
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    firefly luciferase
  • Species
  • Insert Size (bp)
  • Mutation
    destabilizing sequences (DS) added to the C terminus of the protein (CL1 and PEST) and five consecutive AUUUA elements added to the 3′ UTR (RNA DS ARE) for rapid mRNA turnover
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
  • Articles Citing this Plasmid

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-LUC2CP/ARE was a gift from Gideon Dreyfuss (Addgene plasmid # 62857 ; ; RRID:Addgene_62857)
  • For your References section:

    Rapid-response splicing reporter screens identify differential regulators of constitutive and alternative splicing. Younis I, Berg M, Kaida D, Dittmar K, Wang C, Dreyfuss G. Mol Cell Biol. 2010 Apr;30(7):1718-28. doi: 10.1128/MCB.01301-09. Epub 2010 Feb 1. 10.1128/MCB.01301-09 PubMed 20123975