318,246 results

1. pRDA174_EF1a_EnAsCas12a_BFP

   Plasmid

   #196724

   Purpose

   Sortable and selectable EnAsCas12a.

   Depositor

   Bernhard Schmierer

   Insert

   enASCas12a-HA-T2A-BSD-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS - HA and SV40 NLS

   Mutation

   E174R, S542R, K548R

   Promoter

   EF1a

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLifeAct-mScarlet3_N1

   Plasmid

   #189767

   Purpose

   Construct for labelling actin filaments in mammalian cells with lifeact-mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   LifeAct-mScarlet3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-Cre:GFP

   Plasmid

   #13776

   Purpose

   Mammalian expression of Cre recombinase fused to GFP

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Cre-GFP fusion protein
   

   Use

   Cre/Lox

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-RSET-iGABASnFR2-6xHis

   Plasmid

   #218885

   Purpose

   Bacterial expression of improved GABA sensor (positive change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S99A F102Y F104Y L178S

   Promoter

   T7

   Available Since

   May 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-mCherry-IRES-Cre (AAV8)

   Viral Prep

   #55632-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-Ef1a-mCherry-IRES-Cre (#55632). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry-IRES-Cre plasmid DNA. Cre and bicistronic (IRES) mCherry expression under the EF1a promoter. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Promoter

   EF1a and Ef1a/IRES

   Tags

   mCherry

   Available Since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pTD-NTwinStrep_Km

   Plasmid

   #45938

   Purpose

   use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Tags

   6xHis and Twin-Strep

   Expression

   Bacterial

   Promoter

   Trc (lactose/IPTG inducible)

   Available Since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. Mitochondrial cpGFP

   Plasmid

   #215711

   Purpose

   Expresses cpGFP in the mitochondria

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   cpGFP
   

   Use

   Lentiviral

   Tags

   FLAG, HA, and mitochondrial localization sequence…

   Expression

   Mammalian

   Mutation

   circularly permuted GFP

   Promoter

   CMV

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF5B-FRT-GFP-αTAT1

   Plasmid

   #27099

   Depositor

   Maxence Nachury

   Article

   Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

   Insert

   α-Tubulin K40 acetyltransferase1 (ATAT1 Human)
   

   Tags

   GFP, S-tag, and TEV site

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTCF236 HLA-B*40:01

   Plasmid

   #180463

   Purpose

   E. coli expression of soluble MHC protein

   Depositor

   John Altman

   Insert

   HLA-B*40:01
   

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   Replacement of transmembrane domain with enzymati…

   Available Since

   Feb. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC18T-mini-Tn7T-Tp-dsRedExpress

    Plasmid

    #65036

    Purpose

    TpR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    dsRedExpress
    

    Expression

    Bacterial

    Available Since

    Aug. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. GFP-ING2(pHD)

    Plasmid

    #21589

    Depositor

    Junying Yuan

    Article

    Gozani et al Cell. 2003 Jul 11. 114(1):99-111.

    Insert

    GFP-ING2(pHD) (Ing2 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains Ing2 plant homeodomain (PHD) amino acids…

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pAAV.CAG.GCaMP6f.WPRE.SV40

    Plasmid

    #100836

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV2, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    CAG

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.CAG.GCaMP6f.WPRE.SV40

    Plasmid

    #100836

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV2, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    CAG

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX458-AAVS1

    Plasmid

    #113194

    Purpose

    Encodes gRNA for human AAVS1

    Depositor

    Adam Karpf

    Insert

    AAVS1 (PPP1R12C, AAVS1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pE-FLP

    Plasmid

    #45978

    Purpose

    Expresses yeast Flp driven by the constitutive promoter pE from phage P2

    Depositor

    Keith Shearwin, Drew Endy

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Insert

    flp
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    flp driven by the constitutive promoter pE from p…

    Promoter

    pE (from phage P2)

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLG004 (RT-Abi-P2)

    Plasmid

    #157882

    Purpose

    Expresses the RT-Abi-P2 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    RT-Abi-P2
    

    Expression

    Bacterial

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-G-Creon shRNA[Control]

    Plasmid

    #181824

    Purpose

    This vector simultaneously expresses GFP protein and shRNA in a Cre-dependent manner.

    Depositor

    Eun Mi Hwang

    Article

    Kim et al J Neurosci Methods. 2021 Dec 21:109452.

    Type

    Empty backbone

    Use

    AAV and RNAi

    Expression

    Mammalian

    Promoter

    EF1a/U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR_Gal4UAS_Blinatumomab_PGK_mCherry

    Plasmid

    #85437

    Purpose

    [Edit] Lentiviral vector for Gal4 inducible Blinatumomab and constitutive mCherry expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    Blinatumomab
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpyCatcher003

    Plasmid

    #133447

    Purpose

    Expresses SpyCatcher003 in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyCatcher003
    

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. ratGlp1-R

    Plasmid

    #14944

    Depositor

    Bernard Thorens

    Article

    Thorens et al Proc Natl Acad Sci U S A. 1992 Sep 15. 8

    Insert

    Glp1-R (Glp1r Rat)
    

    Expression

    Mammalian

    Available Since

    June 7, 2007

    Availability

    Academic Institutions and Nonprofits only