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TRE-KRAB-dCas9-IRES-BFP

Plasmid

#85449

Purpose

Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP

Depositor

Eric Lander

Publication

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

KRAB-dCas9-IRES-BFP

Use

CRISPR and Lentiviral

Tags

2x NLS, HA Tag, and KRAB domain

Expression

Mammalian

Promoter

TRE3G

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCAG-dCas9-5xPlat2AflD

Plasmid

#82560

Purpose

Expression vector for Cas9 peptide array (linker length: 22aa).

Depositor

Izuho Hatada

Publication

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-5xPlat2AflD

Expression

Mammalian

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pJL-TRBO

Plasmid

#80082

Purpose

An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence.

Depositor

John Lindbo

Publication

Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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CRISPRainbow Kit for Multiplex Labeling

Plasmid kit

#1000000079

Purpose

Plasmids using dCas9 and gRNA scaffolds that bind fluorescent proteins to label multiple chromosomal loci.

Depositor

Thoru Pederson

Application

Genome Editing

Vector type

Lentiviral, Mammalian Expression

Editing type

CRISPR

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
pCRISPRyl

Plasmid

#70007

Purpose

CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

Depositor

Ian Wheeldon

Publication

Schwartz et al ACS Synth Biol. 2015 Dec 29.

Inserts

Codon optimized Cas9 from S. pyogenes
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

SCR1'-tRNA and UAS1B8-TEF(136)

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pCRISPRyl

Plasmid

#70007

Purpose

CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

Depositor

Ian Wheeldon

Publication

Schwartz et al ACS Synth Biol. 2015 Dec 29.

Inserts

Codon optimized Cas9 from S. pyogenes
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

SCR1'-tRNA and UAS1B8-TEF(136)

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pAAVS1-TLR targeting vector

Plasmid

#64215

Purpose

Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct

Depositor

Ralf Kuehn

Publication

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Insert

traffic light reporter (TLR) (AAVS1 Human)

Use

Human targeting

Mutation

see publication for details

Promoter

CAG

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pAAVS1-TLR targeting vector

Plasmid

#64215

Purpose

Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct

Depositor

Ralf Kuehn

Publication

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Insert

traffic light reporter (TLR) (AAVS1 Human)

Use

Human targeting

Mutation

see publication for details

Promoter

CAG

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pMF230

Plasmid

#62546

Purpose

Broad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.

Depositor

Michael Franklin

Publication

Nivens et al J Bacteriol. 2001 Feb;183(3):1047-57.

Insert

GFP mut2

Use

Broad host range plasmid

Promoter

trc

Available since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pMF230

Plasmid

#62546

Purpose

Broad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.

Depositor

Michael Franklin

Publication

Nivens et al J Bacteriol. 2001 Feb;183(3):1047-57.

Insert

GFP mut2

Use

Broad host range plasmid

Promoter

trc

Available since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pQFlag-USP7 WT puroR

Plasmid

#46751

Purpose

Retroviral vector that expresses wild type Flag-tagged human USP7

Depositor

Goedele Maertens, Gordon Peters

Publication

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pQFlag-USP7 WT puroR

Plasmid

#46751

Purpose

Retroviral vector that expresses wild type Flag-tagged human USP7

Depositor

Goedele Maertens, Gordon Peters

Publication

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLL7.0: tgRFPt-SSPB R73Q

Plasmid

#60416

Purpose

Mammalian Expression of tgRFPt-Micro

Depositor

Brian Kuhlman

Publication

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

tgRFPt-SSPB R73Q

Use

Cre/Lox and Lentiviral

Tags

TagRFPt

Expression

Mammalian

Mutation

R73Q, Y11K and A15E

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pLL7.0: tgRFPt-SSPB R73Q

Plasmid

#60416

Purpose

Mammalian Expression of tgRFPt-Micro

Depositor

Brian Kuhlman

Publication

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

tgRFPt-SSPB R73Q

Use

Cre/Lox and Lentiviral

Tags

TagRFPt

Expression

Mammalian

Mutation

R73Q, Y11K and A15E

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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GA-NES

Plasmid

#61018

Purpose

ddGFP A-NES

Depositor

Robert Campbell

Publication

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ddGFP A

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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GA-NES

Plasmid

#61018

Purpose

ddGFP A-NES

Depositor

Robert Campbell

Publication

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ddGFP A

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-TOMM20-N-10

Plasmid

#55328

Purpose

Localization: Outer Mitochondrial Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Publication

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TOMM20 (TOMM20 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

7 Articles

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mTagBFP2-TOMM20-N-10

Plasmid

#55328

Purpose

Localization: Outer Mitochondrial Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Publication

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TOMM20 (TOMM20 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

7 Articles

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mCherry-Rab5a-7

Plasmid

#55126

Purpose

Localization: Ras GTPase, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab5a

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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mCherry-Rab5a-7

Plasmid

#55126

Purpose

Localization: Ras GTPase, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab5a

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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