171,154 results
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Plasmid#127173PurposeEntry plasmid for guide cloningDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable SinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only
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pAAV-CMV-FLEX-SaCas9-U6-sgRosa26
Plasmid#159914PurposeMutagenesis of Rosa26 with SauCas9DepositorInsertRosa26 gRNA (Gt(ROSA)26Sor Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-BICDN-CIBN
Plasmid#102254PurposeExpresses fusion of dynein adapter BICDN (1-594) with GFP and CIB1 (1-170)DepositorAvailable SinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGW-TurboID-GOI-GFP
Plasmid#209383PurposeGateway compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneExpressionPlantMutationTurboID gene sequence C249AAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma12-GFP2
Plasmid#166781PurposeEncodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGGamma12-GFP2 (GNG12 Synthetic, Human)
ExpressionMammalianMutationContains an N-terminal GFP2 and GSAG linkerPromoterCMVAvailable SinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pABE8e-protein
Plasmid#161788PurposeRhamnose-inducible expression of 8xHIS-ABE8e in E. coliDepositorInsertABE8e base editor
Tags8xHIS and BPNLSExpressionBacterialPromoterpRhaBADAvailable SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-EGFP-mir30(Scn9a-scrambled)
Plasmid#79671PurposeScrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)DepositorInsertEGFP
UseAAV and Cre/LoxExpressionMammalianPromoterCAGAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZIF-eGFP-HoTag6
Plasmid#208745Purposepart B of SparkDropDepositorInsertZIF-eGFP-HoTag6
ExpressionMammalianAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Hygro-dTomato
Plasmid#99376PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-dTomato
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1a and hU6Available SinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma5-GFP2
Plasmid#166777PurposeEncodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGGamma5-GFP2 (GNG5 Synthetic, Human)
ExpressionMammalianMutationContains an N-terminal GFP2 and GSAG linkerPromoterCMVAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma3-GFP2
Plasmid#166775PurposeEncodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGGamma3-GFP2 (GNG3 Synthetic, Human)
ExpressionMammalianMutationContains an N-terminal GFP2 and GSAG linkerPromoterCMVAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1
Plasmid#193942Purposea fully murine CAR targeting CD105 (endoglin), a component of the TGFβ receptor expressed on the surface of certain solid tumors and acute leukemias.DepositorInsertheavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv)
UseRetroviralAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
phage-ubc-nls-ha-tdPCP-gfp
Plasmid#40650DepositorInserttdPCP-gfp
UseLentiviralTagsEGFP, HA, and NLSExpressionMammalianPromoterhuman ubiquitin C (UBC)Available SinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMGS7 (AAVS1sgRNA)
Plasmid#126582PurposeA CRISPR/Cas9 construct to target the AAVS1 locus in humansDepositorInsertAAVS1 sgRNA
ExpressionMammalianAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-PIP5K1A
Plasmid#202720Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertEGFP:PIP5K1A (PIP5K1A Human)
ExpressionMammalianAvailable SinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLT61
Plasmid#1690DepositorTypeEmpty backboneUseRNAiTagsT7p, unc224616, unc2218148, unc2218082, unc223827…ExpressionWormAvailable SinceMarch 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pUbC-nls-ha-stdMCP-stdGFP
Plasmid#98916Purposesynonymized tandem dimer MCP fused to synonymized tandem dimer GFPDepositorInsertstdMCP-stdGFP
UseLentiviralTagsGFPPromoterUbCAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE
Plasmid#122151PurposeExpresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.DepositorInsertdCas9 fused to SunTag(24x)
UseLentiviralTags2xNLS-P2A-BFP and NLSExpressionMammalianPromoterSFFVAvailable SinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-G-GECO1.1
Plasmid#32445PurposeMammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.1)DepositorInsertG-GECO1.1
ExpressionMammalianMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…PromoterCMVAvailable SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGH8 - pRAB-3::mCherry::unc-54utr
Plasmid#19359DepositorArticleInsertPrab-3::mCherry
TagsmCherryExpressionWormAvailable SinceOct. 29, 2008AvailabilityAcademic Institutions and Nonprofits only