181,923 results
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Plasmid#137879PurposeDonor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFPDepositorInsertTRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
UseAAVExpressionMammalianAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
R4pGWB601_UBQ10p-Turbo-YFP-NLS
Plasmid#127368PurposeBinary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plantsDepositorInsertTurboID (BirA mutant)
TagsGS linker, NLS, V5, and YFPExpressionPlantMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterArabidopsis UBQ10 promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLifeAct-miRFP670nano
Plasmid#127434PurposeF-Actin labeling with near-infrared fluorescent protein miRFP670nanoDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLifeAct-miRFP670nano
TagsmiRFP670nanoExpressionMammalianPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-mCherry-IRES-Flpo (AAV1)
Viral prep#55634-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-EF1a-mCherry-IRES-Flpo (#55634). In addition to the viral particles, you will also receive purified pAAV-EF1a-mCherry-IRES-Flpo plasmid DNA. EF1a-driven mCherry and bicistronic Flp recombinase expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1a and Ef1a/IRESTagsmCherryAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn-FLEX.iGABASnFR (AAV1)
Viral prep#112163-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.hSyn-FLEX.iGABASnFR (#112163). In addition to the viral particles, you will also receive purified pAAV.hSyn-FLEX.iGABASnFR plasmid DNA. Synapsin-driven, Cre-dependent iGABASnFR GABA sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac2.0
Plasmid#119872PurposeCumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR
sfGFP
UseSynthetic BiologyExpressionBacterialPromoterPveg promoter with CuO sequence and PxylR promoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZS157 CRISPEY RT/Cas9
Plasmid#114454PurposeYeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expressionDepositorInsertSpCas9, Ec86-RT
UseCRISPRTagsSV40-NLSExpressionYeastMutationS. cerevisiae codon optimizedPromoterGAL1-GAL10Available sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTRE-8XGTIIC-DsRED-DD
Plasmid#115798Purposelentiviral, trimethoprim (TMP) regulated YAP promoter activity reporterDepositorAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB_DA1m
Plasmid#113049Purposeexpress the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1m in neuronsDepositorHas serviceAAV9InsertGPCR activation based DA sensor GRAB_DA1m (DRD2 Human)
UseAAVPromoterhSynAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Fuw-dCas9-dead Tet1CD-P2A-BFP
Plasmid#108246PurposeLentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFPDepositorInsertdCas9-dead Tet1CD-HA-P2A-BFP
UseLentiviralTagsHAExpressionMammalianMutationdCas9-D10A, dCas9-H840A, Tet1CD-H1672Y, Tet1CD-D1…PromoterUbcAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Ef1a-DIO ChETA-EYFP (AAV9)
Viral prep#26968-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-Ef1a-DIO ChETA-EYFP (#26968). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO ChETA-EYFP plasmid DNA. EF1a-driven, Cre-dependent, ChETA fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEYFP (Cre-dependent)Available sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AviTag-SnoopLigase
Plasmid#105626PurposePlasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylationDepositorInsertAviTag-SnoopLigase
ExpressionBacterialPromoterT7Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJK02
Plasmid#105133PurposeClostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriTDepositorInsertsUpstream & Downstream pyrE deletion region
tetR
Cas9
gRNA
UseE. coli - c. difficile shuttle vectorAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0039-CMV-dPspCas13b-GS-ADAR2DD(E488Q)
Plasmid#103849PurposedPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseCRISPRTagsGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…PromoterCMVAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lenti-EF1a-dCas9-VP64-Puro
Plasmid#99371Purpose3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)DepositorInsertdCas9-VP64-T2A-Puro
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1aAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
htrpC6-YFP
Plasmid#21084DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTrpC6 (TRPC6 Human)
TagsYFPExpressionMammalianAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTubb3-MC
Plasmid#87112Purposeminicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITIDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP
UseCRISPRAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP
Plasmid#86827PurposeExpresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAsDepositorInsertFLAG-NLS-MS2-eGFP
ExpressionMammalianPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-sfGFP 150TAG
Plasmid#85493PurposepET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150DepositorInsertsuperfolder GFP 150TAG
ExpressionBacterialMutation150 TAG (amino acid 150 mutated to stop codon)PromoterT7Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCIpA102-G-HLA-A2_GFP
Plasmid#85162Purposefor mRNA expression of HLA.A2-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHLA.A2 (HLA-A Human)
UseMrna expressionTagsEGFPExpressionMammalianPromoterCMVAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by