169,065 results

1. pBT281

   Plasmid

   #122611

   Purpose

   express evoAPOBEC1-BE4max in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   evoAPOBEC1-BE4max
   

   Expression

   Mammalian

   Mutation

   E4K H109N H122L D124N R154H A165S P201S F205S

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBA904

   Plasmid

   #122238

   Purpose

   Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.

   Depositor

   Jonathan Weissman

   Article

   Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

   Insert

   sgRNA with cs1 in stem loop 2 of the constant region
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMK347 (BSD-P2A-mAID)

   Plasmid

   #121181

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   BSD-P2A-mAID
   

   Use

   CRISPR

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-iGlucoSnFR-TS

   Plasmid

   #127952

   Purpose

   Expresses the iGlucoSnFR-TS biosensor for intracellular [glucose], with a fluorescence-lifetime readout, in mammalian cells

   Depositor

   Gary Yellen

   Article

   Diaz-Garcia et al J Neurosci Res. 2019 May 20. doi: 10.100

   Insert

   iGlucoSnFR-TS
   

   Use

   AAV

   Tags

   His6 and Xpress epitope

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-mNG-IRES-mScarlet-dPCP

   Plasmid

   #231090

   Purpose

   Bicistronic construct encoding mNG in combination with mScarlet-dPCP via an EMCV-IRES sequence.

   Depositor

   John Ngo

   Article

   Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

   Insert

   mNG-IRES-mScarlet-dPCP
   

   Expression

   Mammalian

   Available Since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pECJ3

   Plasmid

   #75465

   Purpose

   Toggle switch plasmid containing lacI-pdt3

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   lacI-pdt3
   

   Expression

   Bacterial

   Promoter

   pLtetO

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV6M-Pak1

   Plasmid

   #12209

   Depositor

   Jonathan Chernoff

   Article

   Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

   Insert

   Pak1 (PAK1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDL17

   Plasmid

   #106483

   Purpose

   pDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosome

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

   Inserts

   gam
   bet
   exo
   lacI
   rhaR
   rhaS
   

   Expression

   Bacterial

   Available Since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. RKS-Fzd7/8 subtype NGS Wnt

   Plasmid

   #159628

   Purpose

   Express Fzd7/8 subtype NGS Wnt in mammalian cells

   Depositor

   Chris Garcia

   Article

   Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

   Insert

   Fzd7/8 subtype NGS Wnt
   

   Use

   Lentiviral

   Tags

   His tag

   Expression

   Mammalian

   Available Since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3

    Plasmid

    #127356

    Purpose

    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and mVenus

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Joint Modular Cloning (JMC) Toolkit

    Plasmid Kit

    #1000000233

    Purpose

    The Joint Modular Cloning (JMC) Toolkit includes a modular cloning chassis and set of functional parts for assembling plant transformation vectors.

    Depositor

    Daniel Voytas

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

12. LentiCRISPRv2-Opti

    Plasmid

    #163126

    Purpose

    LentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEcCas-2.0

    Plasmid

    #190697

    Purpose

    Constitutive expressing of Cas9 and inducible expression of lambda Red

    Depositor

    Sheng Yang

    Article

    Li et al ACS Synth Biol. 2023 Mar 3. doi: 10.1021

    Insert

    Cas9 and Lamda Red system
    

    Expression

    Bacterial

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28sumo-MDA5-Full-length

    Plasmid

    #210826

    Purpose

    protein expression in ecoli

    Depositor

    Anna Pyle

    Article

    Fitzgerald et al Nucleic Acids Res. 2014 Apr;42(6):3919-3

    Insert

    MDA5 (IFIH1) Full Length Protein (IFIH1 Human)
    

    Expression

    Bacterial

    Mutation

    NA

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRN110

    Plasmid

    #84462

    Purpose

    pJB38-NWMN29-30 + SarA_P1-mKate2-Term

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    SarA_P1-mKate2 from pRN11
    

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. LAMP1-APEX2

    Plasmid

    #215555

    Purpose

    Stable, inducible expression in lysosome

    Depositor

    Nevan Krogan

    Article

    Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

    Insert

    LAMP1-EGFP-Flag tag-APEX2-NES
    

    Use

    Lentiviral

    Available Since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. EB1-tdTomato

    Plasmid

    #50825

    Purpose

    expresses EB1-tdTomato fusion in mammalian cells for labeling the growing tips of MTs

    Depositor

    Erik Dent

    Insert

    EB1 (Mapre1 Mouse)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5/FRT/TO V5 HSPA1L

    Plasmid

    #19511

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 1-like (HSPA1L Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Ef1a-fDIO mCherry

    Plasmid

    #114471

    Purpose

    Flp-dependent mCherry Control

    Depositor

    Karl Deisseroth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    mCherry
    

    Use

    AAV

    Promoter

    Ef1a

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCFD1-dU6:1gRNA

    Plasmid

    #49408

    Purpose

    expression of gRNA under control of the Drosophila U6:1 promoter

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    dU6-1:gRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    dU6-1

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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