317,434 results

1. pSW002-Pc-mRuby2

   Plasmid

   #111250

   Purpose

   Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mRuby2 (codon optimized for P. fluorescens)

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   mRuby2
   

   Expression

   Bacterial

   Mutation

   mRuby2 is codon optimized for expression in P. fl…

   Promoter

   Pc

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR5'_ubi

   Plasmid

   #27320

   Depositor

   Leonard Zon

   Article

   Mosimann et al Development. 2011 Jan . 138(1):169-77.

   Insert

   zebrafish ubiquitin promoter (ubb Zebrafish)
   

   Use

   Multisite gateway 5' entry vector

   Available Since

   Feb. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. mscv2.2-NLRC4

   Plasmid

   #60199

   Purpose

   Mouse NLRC4 expressed under the constitutive, moderate-level retroviral LTR promoter, expresses in mammalian cells; IRES-GFP reporter downstream of NLRC4

   Depositor

   Russell Vance

   Article

   Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

   Insert

   NLRC4 (Nlrc4 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-PH-TAPP1

   Plasmid

   #161985

   Purpose

   Expresses GFP-tagged PH-Tapp1 in mammalian cells (PI(3,4)P2 biosensor)

   Depositor

   David Bryant, Dario Alessi

   Article

   Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

   Insert

   PH-TAPP1 (PLEKHA1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

   Plasmid

   #199390

   Purpose

   Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   anti-ALFA nanobody
   

   Tags

   14xHis-Avi-SUMOEu

   Expression

   Bacterial

   Promoter

   T5-LacO

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. vHB8

   Plasmid

   #193454

   Purpose

   Expression of GFP-Cas9-NLS and gRNA in A. castellanii

   Depositor

   Chantal Abergel

   Article

   Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

   Insert

   GFP-Cas9-NLS
   

   Use

   CRISPR

   Tags

   GFP

   Promoter

   GAPDH

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TRE-UBC-hCD47-T2A-GFP

   Plasmid

   #205458

   Purpose

   lentiviral plasmid for expression of human CD47

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   hCD47 (CD47 Human)
   

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA

   Plasmid

   #156445

   Purpose

   For transient expression of mouse RAD21 (SCC1) tagged with mKate2

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   mouse RAD21 (Rad21 Mouse)
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 deltaN89 Beta-catenin

   Plasmid

   #19288

   Depositor

   Eric Fearon

   Article

   Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

   Insert

   beta-catenin (CTNNB1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N-terminal truncation, deleted aa 1-89

   Available Since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

10. paavCAG-JxON-pre-mGRASP-mCerulean

    Plasmid

    #51903

    Purpose

    JxOn-pre

    Depositor

    Jinhyun Kim

    Article

    Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

    Insert

    presynaptic mGRASP
    

    Use

    AAV and Cre/Lox

    Promoter

    CAG

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. eMscL WT

    Plasmid

    #107454

    Purpose

    eMscL WT is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif

    Depositor

    Francesco Difato

    Article

    Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393

    Insert

    bacterial mechanosensitive ion channel of large conductance (mscL Escherichia Coli)
    

    Use

    Adeno-associated viral

    Tags

    Kir2.1 ER export signal (FCYENEV) and tdTomato fl…

    Expression

    Mammalian

    Promoter

    human synapsin 1

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWZL Blast myc

    Plasmid

    #10674

    Depositor

    William Hahn

    Article

    Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

    Insert

    c-myc (MYC Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCROPseq-VEX-optimized-scaffold

    Plasmid

    #203321

    Purpose

    Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-synPCB2.1

    Plasmid

    #139478

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    MTS-Fnr-Fd-P2A-MTS-PcyA-FLAG-P2A-MTS-HA-HO1
    

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLacO-pEF1α-RFP

    Plasmid

    #179507

    Purpose

    Expresses monomeric RFP in mammalian cells under a EF1α promoter

    Depositor

    Yasushi Hiraoka

    Article

    Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

    Insert

    monomeric RFP
    

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330 Pten

    Plasmid

    #59909

    Purpose

    pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    sgRNA targeting Pten (Pten Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTRIPZ

    Plasmid

    #206981

    Purpose

    empty pTRIPZ vector for cloning of doxycycline-inducible gene expression

    Depositor

    Ming Chen

    Article

    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. SacI ML GFP Strand 11 Short

    Plasmid

    #164121

    Purpose

    Detect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

    Depositor

    Tito Calì, Marisa Brini

    Article

    Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

    Insert

    SacI ML GFP Strand 11 Short
    

    Expression

    Mammalian

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid

    #107941

    Purpose

    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a (E174R/S542R/K548R)
    

    Tags

    3x HA and NLS (nucleoplasmin)

    Expression

    Mammalian

    Mutation

    E174R, S542R and K548R

    Promoter

    CAG

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. NLS-LgBiT-FRB

    Plasmid

    #183403

    Purpose

    Expresses nuclear-localized LgBiT-FRB

    Depositor

    Alice Ting

    Article

    Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

    Insert

    LgBiT-FRB
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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