Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pCINeo-myc-Itch (C822S)/2860
(Plasmid #11428)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11428 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCI-Neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Itch
  • Alt name
    Itchy
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2500
  • Mutation
    cysteine at 822 mutated to serine
  • GenBank ID
    AF037454
  • Entrez Gene
    Itch (a.k.a. 6720481N21Rik, 8030492O04Rik, A130065M08, AIP4, C230047C07Rik)
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe I (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer T7 (modified) 5'-TAATACGACTCACTATAGG-3'
  • 3′ sequencing primer T3 (common)
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Neal Copeland/NCI
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCINeo-myc-Itch (C822S)/2860 was a gift from Allan Weissman (Addgene plasmid # 11428 ; http://n2t.net/addgene:11428 ; RRID:Addgene_11428)
  • For your References section:

    WW domain HECT E3s target Cbl RING finger E3s for proteasomal degradation. Magnifico A, Ettenberg S, Yang C, Mariano J, Tiwari S, Fang S, Lipkowitz S, Weissman AM. J Biol Chem. 2003 Oct 31. 278(44):43169-77. 10.1074/jbc.M308009200 PubMed 12907674