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pCINeo-myc-Itch (C822S)/2860
(Plasmid #11428)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11428 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 11428-D50 50 μg of DNA in Tris buffer $495
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DNA 11428-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCI-Neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Itch
  • Alt name
    Itchy
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2500
  • Mutation
    cysteine at 822 mutated to serine
  • GenBank ID
    AF037454
  • Entrez Gene
    Itch (a.k.a. 6720481N21Rik, 8030492O04Rik, A130065M08, AIP4, C230047C07Rik)
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe I (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer T7 (modified) 5'-TAATACGACTCACTATAGG-3'
  • 3′ sequencing primer T3 (common)
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Neal Copeland/NCI

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCINeo-myc-Itch (C822S)/2860 was a gift from Allan Weissman (Addgene plasmid # 11428 ; http://n2t.net/addgene:11428 ; RRID:Addgene_11428)

  • For your References section:

    WW domain HECT E3s target Cbl RING finger E3s for proteasomal degradation. Magnifico A, Ettenberg S, Yang C, Mariano J, Tiwari S, Fang S, Lipkowitz S, Weissman AM. J Biol Chem. 2003 Oct 31. 278(44):43169-77. 10.1074/jbc.M308009200 PubMed 12907674
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