167,318 results

1. pAAV-RAM-d2TTA::TRE-ChR2-WPREpA

   Plasmid

   #84471

   Purpose

   pAAV-RAM-ChR2

   Depositor

   Yingxi Lin

   Article

   Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

   Insert

   Channelrhodopsin 2
   

   Use

   AAV

   Tags

   EYFP

   Mutation

   H134R

   Promoter

   TRE

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF1-alpha-V

   Plasmid

   #27290

   Depositor

   Timothy Springer

   Article

   Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

   Insert

   integrin alpha V (ITGAV Human)
   

   Expression

   Mammalian

   Mutation

   Isoform 2

   Available Since

   March 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1 shRUNX1 puro

   Plasmid

   #45816

   Depositor

   Kevin Janes

   Article

   Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

   Insert

   Runx1 (RUNX1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-dCas13-M3M14nes

   Plasmid

   #155367

   Purpose

   Targeted m6A RNA methylation in mammalian cells

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Mito-V5-BS2 (Mitochondrial matrix)

   Plasmid

   #220344

   Purpose

   Mammalian expression vector BS2 in mitocondria

   Depositor

   Bryan Dickinson

   Article

   Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

   Insert

   BS2
   

   Tags

   MTS-matrix and V5 tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. V5-BS2-SENP2 (Nuclear Pore)

   Plasmid

   #220345

   Purpose

   Mammalian expression vector BS2 in nuclear pore

   Depositor

   Bryan Dickinson

   Article

   Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

   Insert

   BS2
   

   Tags

   SENP2 (nuclear pore localized protein) and V5 tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pcDNA3-RTP1S

   Plasmid

   #149364

   Purpose

   Expresses RTP1S in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Inagaki et al Cell Rep. 2020 Jun 30;31(13):107814. doi

   Insert

   RTP1S (Rtp1 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTNT-pT7-tetR-mCherry

   Plasmid

   #140870

   Purpose

   T7 promoter driven synthesis of TetR-mCherry fusion protein

   Depositor

   Neal Devaraj

   Article

   Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

   Insert

   TetR-mCherry expressed with T7 promoter
   

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2118

   Plasmid

   #141929

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TBPL1 (TBPL1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX855

    Plasmid

    #62887

    Purpose

    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Aspartic acide 10 to Alanine (D10A)

    Promoter

    CBh

    Available Since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC57-4eCOL2A1

    Plasmid

    #97211

    Purpose

    For cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectors

    Depositor

    Ryan Porter

    Article

    De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

    Insert

    4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
    

    Use

    Vector is designed for cloning and generation of …

    Promoter

    COL2A1 regulatory elements

    Available Since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pADH147

    Plasmid

    #90991

    Purpose

    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    gRNA conserved, FRT, DS_HIS1
    

    Use

    CRISPR

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbA8k-RFP

    Plasmid

    #35273

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BAD

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_IRGM_WT

    Plasmid

    #82134

    Purpose

    Gateway Donor vector containing IRGM , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    IRGM (IRGM Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-C-mScarlet-EFS-Blast

    Plasmid

    #227260

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHM130

    Plasmid

    #225529

    Purpose

    Encodes destablized GFP under IBRE4 promoter, and constitutive BFP

    Depositor

    Jared Toettcher

    Article

    McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

    Inserts

    GFP
    BFP
    

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWL078 MCP-2xGFP

    Plasmid

    #197047

    Purpose

    pCYC-NLS-MCP-2xGFP on pRS305

    Depositor

    Robert Singer

    Article

    Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

    Insert

    MCP-2xGFP
    

    Expression

    Yeast

    Promoter

    CYC1

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-PLXNB2

    Plasmid

    #86237

    Purpose

    Lentivirus for expression of human Plexin-B2

    Depositor

    Roland Friedel

    Article

    Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

    Insert

    Plexin-B2 (PLXNB2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    full length PLXNB2, CRISPR resistant

    Promoter

    human PGK promoter

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-IRF9

    Plasmid

    #71452

    Purpose

    Lentiviral expression of IRF9

    Depositor

    George Stark

    Article

    Cheon et al EMBO J. 2013 Oct 16;32(20):2751-63. doi:

    Insert

    IRF9 (IRF9 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. HBx

    Plasmid

    #65463

    Purpose

    expresses HBx in mammalian cells

    Depositor

    Wang-Shick Ryu

    Article

    Cha et al Hepatology. 2004 Jun;39(6):1683-93.

    Insert

    HBV X protein
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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