179,692 results
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Plasmid#188779PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsZim3 KRAB-NLS fusionPromoterSFFVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
spNW30
Plasmid#173485PurposeExpresses spNW30 in Bl21DepositorInsertspNW30
TagsHistagExpressionBacterialAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_CoV2_501V2
Plasmid#170449PurposeExpress SARS-CoV-2 501V2 (South Africa strain / beta strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 501V2 pseudotyped virus production.DepositorInsertSARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, 501V2 (B.1.351))
TagsNAExpressionMammalianMutationL18F, D80A, D215G, R246I, K417N, E484K, N501Y, D6…PromoterCMVAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.3_CoV2_P1
Plasmid#170450PurposeExpress SARS-CoV-2 P.1 (Brazil / gamma) strain spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 P.1 pseudotyped virus production.DepositorInsertSARS-CoV-2 P.1 spike D18 (S SARS-CoV-2, P.1 strain)
TagsNAExpressionMammalianMutationL18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…PromoterCMVAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV SYN flex PSAM4 GlyR IRES EGFP (AAV9)
Viral prep#119741-AAV9PurposeReady-to-use AAV9 particles produced from AAV SYN flex PSAM4 GlyR IRES EGFP (#119741). In addition to the viral particles, you will also receive purified AAV SYN flex PSAM4 GlyR IRES EGFP plasmid DNA. Synapsin-driven, Cre-dependent expression of PSAM4 GlyR IRES EGFP for neuronal silencing. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP8s-WPRE
Plasmid#162377PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expressionDepositorHas serviceAAV1, AAV9, and AAV RetrogradeInsertjGCaMP8s
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationS26M F286Y Q315HPromoterSynapsinAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-FOXA1
Plasmid#153109PurposeExpression of Flag tagged human FOXA1 in mammalian cellsDepositorAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin
Plasmid#133027PurposeExpresses EGFP-tagged late endosome markerDepositorInsertRAB7A (RAB7A Human)
UseLentiviralTagsEGFPExpressionMammalianPromoterHuman elongation factor 1 alphaAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pORANGE Dlg4-GFP KI
Plasmid#131477PurposeEndogenous tagging of PSD95: C-terminal (amino acid position: R721)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSH-EFIRES-P-AtAFB2
Plasmid#129715PurposeAAVS1 safe harbor targeting vector expressing AtAFB2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAFB2 (AFB2 Mustard Weed)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsnoExpressionMammalianMutationcodon optimizedPromoterEF1aAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
amilCP chromoprotein
Plasmid#117847PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coliDepositorInsertpromoter, RBS, amilCP
UseSynthetic Biology; Escherichia coliAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-mCherry-IRES-Flpo (AAV1)
Viral prep#55634-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-EF1a-mCherry-IRES-Flpo (#55634). In addition to the viral particles, you will also receive purified pAAV-EF1a-mCherry-IRES-Flpo plasmid DNA. EF1a-driven mCherry and bicistronic Flp recombinase expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1a and Ef1a/IRESTagsmCherryAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG PSAM4-5HT3 HC IRES EGFP
Plasmid#119740PurposeChemogenetic activator expression plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianMutationL131G, Q139L, Y217F, R420Q, R424D, R428APromoterCAGAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry- SEC61B
Plasmid#121160PurposeMammalian expression of mCherry tagged SEC61B, used as a general ER markerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSEC61B (SEC61B )
ExpressionMammalianAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTRE-8XGTIIC-DsRED-DD
Plasmid#115798Purposelentiviral, trimethoprim (TMP) regulated YAP promoter activity reporterDepositorAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB_DA1m
Plasmid#113049Purposeexpress the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1m in neuronsDepositorHas serviceAAV9InsertGPCR activation based DA sensor GRAB_DA1m (DRD2 Human)
UseAAVPromoterhSynAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCT310
Plasmid#111140PurposeBacterial expression of 6His-dCas9-3XFlagDepositorInsertdCas9
Tags3X Flag and 6X HisExpressionBacterialMutationD10A - H840APromoterT7Available sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-GFP
Plasmid#106281PurposeA version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.DepositorInsertCMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
UseCRISPR and LentiviralExpressionMammalianPromoterCMVAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ELONGIN BC (XX01TCEB1A-c001)
Plasmid#110274PurposeElongin B and Elongin C co-expression plasmid for structure determination; Full length Elongin B & amino acids 17-112 of Elogin CDepositorExpressionBacterialMutationElongin B is full length. Elongin C contains a.a…PromoterT7Available sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Ef1a-DIO ChETA-EYFP (AAV9)
Viral prep#26968-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-Ef1a-DIO ChETA-EYFP (#26968). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO ChETA-EYFP plasmid DNA. EF1a-driven, Cre-dependent, ChETA fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEYFP (Cre-dependent)Available sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only