225,103 results

1. pEGFP-N1-human lyn(C468A)–GFP

   Plasmid

   #35959

   Purpose

   Expresses human lyn protein codon optimized for zebrafish expression.

   Depositor

   Anna Huttenlocher

   Article

   Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

   Insert

   LYN (LYN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   c468a

   Promoter

   cmv

   Available since

   April 27, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pTn7-SCOUT21.RB

   Plasmid

   #201007

   Purpose

   pLv1-pUC18R6KT-miniTn7-Gm-mCherry-TagBFP, GmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Inserts

   mcherry
   TagBFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23104

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmScarlet-HSP70

   Plasmid

   #163790

   Purpose

   Expresses mScarlet-Hsp70 in mammalian cells

   Depositor

   Vincent Timmerman

   Article

   Adriaenssens et al Sci Rep. 2020 May 29;10(1):8755. doi: 10

   Insert

   HSP70 (HSPA1A Human)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET C-terminal TEV His6 cloning vector with BioBrick polycistronic restriction sites (9Bc)

   Plasmid

   #48285

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIYC04

   Plasmid

   #64741

   Purpose

   Yeast expression plasmid

   Depositor

   Jens Nielsen

   Article

   Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

   Plasmid

   #199252

   Purpose

   Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   High specificity eSpCas9(1.1)-D10A nickase
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A K848A K1003A R1060A

   Promoter

   CAG promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCRISPRv2 Neo sgTREX1

   Plasmid

   #127645

   Purpose

   Knock-out of human TREX1 with NeoR

   Depositor

   Nicolas Manel

   Article

   Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

   Insert

   TREX1 sgRNA (TREX1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE-60NA-msGFP2

   Plasmid

   #135301

   Purpose

   Encodes a photostable monomeric superfolder GFP

   Depositor

   Benjamin Glick

   Article

   Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

   Insert

   msGFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. A*11:01:01:01

   Plasmid

   #203276

   Purpose

   Overexpress HLA allele

   Depositor

   Derin Keskin, Catherine Wu

   Article

   Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

   Insert

   A*11:01:01:01 (HLA-A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

    Plasmid

    #185916

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8.20m-nSpG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCpfSA

    Plasmid

    #187890

    Purpose

    CRISPR/Cpf1-based Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus

    Depositor

    Quanjiang Ji

    Article

    Wang et al ACS Synth Biol. 2022 Sep 16;11(9):3049-3

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CLYBL_Bxb1-GA_LP_Dual_TC

    Plasmid

    #229776

    Purpose

    CLYBL targeting construct with the Bxb1-GA landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    FRT-mScarlet-attP(GA)-PuroR-F3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOCC102-His-MBP-DDX3X(WT)

    Plasmid

    #219988

    Purpose

    DDX3X(WT) protein purification

    Depositor

    Simon Alberti

    Insert

    DDX3X(WT) (DDX3X Human)
    

    Use

    Tags

    6xHis and MBP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmGFP-P2A-K(AAA)20-P2A-RFP

    Plasmid

    #105688

    Purpose

    Double fluorescent stalling reporter K(AAA)20

    Depositor

    Ramanujan Hegde

    Article

    Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

    Inserts

    P2A-3xFLAG-VHPbeta
    K(AAA)20
    P2A-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (from vector)

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWPI-IRES-Puro-Ak

    Plasmid

    #154984

    Purpose

    Lentiviral vector for expressing mammalian proteins

    Depositor

    Sonja Best

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC57-mitoLbNOX

    Plasmid

    #74448

    Purpose

    This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

    Insert

    mitoLbNOX
    

    Use

    Tags

    Flag tag with linker and mitochondrial targeting …

    Expression

    Mutation

    Human codon optimized

    Promoter

    Available since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF2533

    Plasmid

    #144341

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX1 (SOX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. hSyn1-eYFP (AAV PHP.eB)

    Viral Prep

    #117382-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Promoter

    Syn

    Tags

    eYFP

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

19. CMV Flag ATRwt

    Plasmid

    #41909

    Depositor

    Stephen Elledge

    Article

    Cortez et al Science. 2001 Nov 23;294(5547):1713-6.

    Insert

    ATR ATR serine/threonine kinase (ATR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SRa

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCasAb-apr

    Plasmid

    #121998

    Purpose

    Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge