168,956 results

1. pcDNA3.1 - HA-KLF4 FL

   Plasmid

   #34593

   Depositor

   Michael Ruppert

   Article

   Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

   Insert

   KLF4 (KLF4 Human)
   

   Tags

   HA tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PIP2-GFP(ST) sensor

   Plasmid

   #170356

   Purpose

   GFP(ST)-PH fusion used to measure realtime changes in PIP2 levels.

   Depositor

   Kees Jalink

   Article

   van der Wal et al J Biol Chem. 2001 May 4. 276(18):15337-4

   Insert

   GFP(ST)-PH (PLCD1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCherry-CD81-10

   Plasmid

   #55012

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   CD81 (CD81 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFastBac1-His10FLAG

   Plasmid

   #157736

   Purpose

   empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

   Depositor

   Debra Hansen

   Article

   Olson et al Protein Expr Purif. 2021 May 7:105890. d

   Type

   Empty backbone

   Tags

   His10 + 3xFLAG, enterokinase-cleavable

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ARR3tk-eGFP/SV40-mCherry

   Plasmid

   #132360

   Purpose

   To measure transcriptional activity of Androgen Receptor

   Depositor

   Charles Sawyers

   Article

   Lee et al Elife. 2019 Jan 15;8. pii: 41913. doi: 1

   Insert

   AR responsive elements (AR Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (AAV Retrograde)

   Viral Prep

   #100853-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (#100853). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven, Cre-dependent GECO1a calcium sensor. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Promoter

   Syn

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pCas9_GFP

   Plasmid

   #44719

   Purpose

   Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Cas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFP Centrin2 (Nigg UK185)

   Plasmid

   #41147

   Depositor

   Erich Nigg

   Article

   Kleylein-Sohn et al Dev Cell. 2007 Aug;13(2):190-202.

   Insert

   Centrin-2 (CETN2 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA4 myc PGC-1 alpha

   Plasmid

   #10974

   Purpose

   Mammalian expression of PGC-1a with myc and his tags

   Depositor

   Toren Finkel

   Article

   Ichida et al J Biol Chem. 2002 Dec 27. 277(52):50991-

   Insert

   PGC-1 alpha (PPARGC1A Human)
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET23b-15F11-HA-mEGFP-6xHis

    Plasmid

    #129593

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    

    Tags

    6xHis-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 15, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. N-Cadherin-EGFP

    Plasmid

    #18870

    Depositor

    Valeri Vasioukhin

    Article

    Nechiporuk et al Dev Cell. 2007 Sep . 13(3):338-50.

    Insert

    N-Cadherin (Cdh2 Mouse)
    

    Tags

    EGFP, Kanamycin, and N-Cadherin

    Expression

    Mammalian

    Available Since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HyPer7.2DAAO-CAV

    Plasmid

    #168303

    Purpose

    Chemogenetic fluorescent H2O2 reporter targeted to the caveolae

    Depositor

    Thomas Michel

    Article

    Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

    Insert

    HyPer7.2DAAO-CAV
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR223_FBXW7_WT

    Plasmid

    #81795

    Purpose

    Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    FBXW7 (FBXW7 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MBP-FUS_FL_WT

    Plasmid

    #98651

    Purpose

    Expresses MBP-tagged full length FUS

    Depositor

    Nicolas Fawzi

    Article

    Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

    Insert

    FUS (FUS Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Available Since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGTetRnls

    Plasmid

    #26599

    Depositor

    Peter Andrews

    Article

    Zafarana et al Stem Cells. 2009 Apr . 27(4):776-82.

    Insert

    Tetracycline repressor (TetR E.Coli)
    

    Tags

    nls

    Expression

    Mammalian

    Available Since

    Nov. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKM208

    Plasmid

    #13077

    Depositor

    Kenan Murphy

    Article

    Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

    Insert

    lacI repressor (lacI E. coli)
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHRm-NLS-dCas9-GFP11x7-NLS

    Plasmid

    #70224

    Purpose

    Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    NLS-dCas9-GFP11x7-NLS
    

    Tags

    GFP11x7

    Expression

    Mammalian

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGWB402-omega

    Plasmid

    #74797

    Purpose

    Gateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    2xCaMV35S enhancer and omega

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLC-ZsGreen-P2A-Puro

    Plasmid

    #124302

    Purpose

    Lentiviral vector for expression of ZsGreen-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    ZsGreen
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-HA AKT1

    Plasmid

    #78778

    Purpose

    To overexpress AKT1 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

    Insert

    AKT1 (AKT1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge