167,318 results

1. pVeBtA2

   Plasmid

   #238987

   Purpose

   Mammalian expression of bovine arrestin-2 long isoform with Venus N-terminal fusion

   Depositor

   Vsevolod Gurevich

   Article

   Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

   Insert

   arrestin-2 long (ARRB2 Bovine)
   

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3VEA3

   Plasmid

   #234796

   Purpose

   Mammalian expression of bovine arrestin-3

   Depositor

   Vsevolod Gurevich

   Article

   Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

   Insert

   Arrestin-3 (ARR3 Bovine)
   

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   none

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMVtrunc F-tractinN9-39-msfGFPΔN7

   Plasmid

   #231552

   Purpose

   Mammalian expression of C-terminally truncated F-tractin fused to N-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   F-tractin (Itpka Rat)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Promoter

   CMVtrunc

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMVtrunc msfGFPΔC9-LifeactΔN2

   Plasmid

   #231556

   Purpose

   Mammalian expression of N-terminally truncated Lifeact fused to C-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   Lifeact (ABP140 Budding Yeast)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Promoter

   CMVtrunc

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKanB

   Plasmid

   #223523

   Purpose

   Used for expression of intracellular recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

   Depositor

   Geoff Lin-Cereghino

   Article

   Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   Sept. 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. P1K-3HA-Atg8∆R-MET15

   Plasmid

   #207034

   Purpose

   Expression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Tags

   3xHA

   Expression

   Yeast

   Mutation

   ∆R

   Promoter

   pATG8

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMZ828

   Plasmid

   #155322

   Purpose

   Constitutive expression of PhyB(1-650)-VP16 in plants

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   PCaMV35S-PhyB(1-650)-VP16-NLS-Tnos
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pROF021

   Plasmid

   #155326

   Purpose

   Expression of PULSE-controlled FLuc

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   (etr)8-(C120)5-PhCMVmin-FLuc-TSV40
   

   Use

   Luciferase and Synthetic Biology; Eukaryotic expr…

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR

   Plasmid

   #206213

   Purpose

   PiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplification

   Depositor

   Masamichi Kamihira

   Article

   Kitano et al Cytotechnology. 2021 Jun;73(3):353-362.

   Insert

   tetracycline-responsive transactivator
   

   Expression

   Mammalian

   Available Since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF2476

    Plasmid

    #144030

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZFHX2 (ZFHX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD649-HAsp-CD70-COMP5AP-AviTag-9xHis

    Plasmid

    #157113

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    CD70 (CD70 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. IRE1-2EGFP-ter-kanMX4

    Plasmid

    #184766

    Purpose

    Integration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    IRE1 (IRE1 Budding Yeast)
    

    Tags

    2EGFP

    Expression

    Yeast

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFRTpsiCHECK_SEAP

    Plasmid

    #154461

    Purpose

    Expression of SEAP and firefly luciferase

    Depositor

    Markus Landthaler

    Article

    Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

    Insert

    SEAP
    

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. H2B-HT-emiRFP703

    Plasmid

    #183985

    Purpose

    Expresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.

    Depositor

    Pablo Rivera-Fuentes

    Article

    Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

    Insert

    H2B (H2BC11 Human)
    

    Tags

    HaloTag, emiRFP703

    Expression

    Mammalian

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-HOXD13(+8A)-IDR-mCherry-CRY2-NLS

    Plasmid

    #145262

    Purpose

    Mammalian Expression of Nuclear Opto HOXD13(+8A) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+8A)-IDR (HOXD13 Human)
    

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-EPHA2-G391R

    Plasmid

    #116320

    Purpose

    Lentiviral expression of EPHA2 G391R

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EPHA2 (EPHA2 Human)
    

    Use

    Lentiviral

    Mutation

    G391R

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGP-AAV-syn-jGCaMP7s-WPRE (AAV1)

    Viral Prep

    #104487-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP7s-WPRE (#104487). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven GCaMP7s calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Promoter

    Syn

    Available Since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pMax_ScaI_BFP

    Plasmid

    #177824

    Purpose

    Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    tagBFP
    

    Expression

    Mammalian

    Mutation

    5' inserted ScaI RE cut site

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Liu Mouse CRISPR Knockout Library in lentiCRISPRv2-blast

    Pooled Library

    #1000000173

    Depositor

    Xiaole Shirley Liu

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

20. GAPDH

    Plasmid

    #155622

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    GAPDH (GAPDH Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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