224,103 results

1. pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

   Plasmid

   #67979

   Purpose

   Cas9 activity reporter (control) with BFP and GFP.

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSDM177

   Plasmid

   #194476

   Purpose

   pHERD30T-crRNA(Lse)_V3

   Depositor

   Joseph Bondy-Denomy

   Article

   Guan et al Nat Microbiol. 2022 Oct 31. pii: 10.1038

   Insert

   crRNA(Lse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCnCre

   Plasmid

   #232907

   Purpose

   Electroporation vector for expression of Cre recombinase in Cuprividus necator

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

   Insert

   Cre recombinase (cre Synthetic)
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Ptac-cymO

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pI3_EGFP

   Plasmid

   #239335

   Purpose

   Expresses the I3-01 nanocage subunit C-terminally tagged with EGFP in mammalian cells. Assembles into nanocages tagged with 60 EGFPs.

   Depositor

   Torsten Wittmann

   Insert

   I3-01
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K129A

   Promoter

   CMV

   Available since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmStayGold_mStayGold_I3

   Plasmid

   #239339

   Purpose

   Expresses the I3-01 nanocage subunit N-terminally tagged with two copies of mStayGold in mammalian cells. Assembles into nanocages tagged with 120 FPs.

   Depositor

   Torsten Wittmann

   Insert

   I3-01
   

   Use

   Tags

   mStayGold

   Expression

   Mammalian

   Mutation

   K129A

   Promoter

   CMV

   Available since

   June 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmScarlet3_I3

   Plasmid

   #239342

   Purpose

   Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3 in mammalian cells. Assembles into nanocages tagged with 60 FPs.

   Depositor

   Torsten Wittmann

   Insert

   I3-01
   

   Use

   Tags

   mScarlet3

   Expression

   Mammalian

   Mutation

   K129A

   Promoter

   CMV

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. fabp10a:ER-CreER; cryaa:mCerulean

   Plasmid

   #230077

   Purpose

   Expression of ERCreER in zebrafish hepatocytes.

   Depositor

   Sumeet Pal Singh

   Article

   Hovhannisyan et al bioRxiv 2025.05.23.655316

   Insert

   ERT2-Cre-ERT2
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   fabp10a

   Available since

   May 30, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAAV hSyn INTRON mCherry-Jph3 WPRE

   Plasmid

   #236237

   Purpose

   AAV expression of human Junctophilin3 N-terminally tagged with mCherry

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   Junctophilin 3 (JPH3 Human)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. SIV_Gag_Pol

   Plasmid

   #236243

   Purpose

   3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Gag and Pol

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   SIV Gag and Pol
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMY.PRC2

    Plasmid

    #234582

    Purpose

    Expression of ProtA-EZH2, SUZ12, EED and RBBP4

    Depositor

    Chen Davidovich

    Article

    Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

    Insert

    EZH2, SUZ12, EED, RBBP4 (EZH2, SUZ12, RBBP4, EED Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pColdI-DDX3_helicase_core

    Plasmid

    #233618

    Purpose

    Expression of His-tagged helicase core region of DDX3X in E.coli

    Depositor

    Shintaro Iwasaki

    Article

    Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

    Insert

    DDX3X helicase core WT (DDX3X Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-S5E6-dTom-nlsdTom (AAV1)

    Viral Prep

    #135641-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-S5E6-dTom-nlsdTom (#135641). In addition to the viral particles, you will also receive purified pAAV-S5E6-dTom-nlsdTom plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato in VIP cortical interneurons under the control of the E6 regulatory element. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Promoter

    Tags

    dTomato

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

13. R2pH-LAMPx3ALFA

    Plasmid

    #225136

    Purpose

    R2pH-LAMP1-labelled lysosomes with 3xLFA

    Depositor

    Massimiliano Stagi

    Article

    Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

    Insert

    Lamp1 (Lamp1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRDB_161

    Plasmid

    #216091

    Purpose

    Cas9 [Sp] CRISPRi targeting CD81, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD81 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. EC67892

    Plasmid

    #209499

    Purpose

    Cloning protospacers for Cas12a editing

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas12a-tRNA-DR-DR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TaU3

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF3460

    Plasmid

    #144936

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PSMB1 (PSMB1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6

    Plasmid

    #177849

    Purpose

    Bacterial Expression of PNR

    Depositor

    Karsten Melcher

    Article

    Tan et al PLoS One. 2013 Sep 12;8(9):e74359. doi:

    Insert

    photoreceptor cell-specific nuclear receptor (NR2E3 Human)
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLCHKO_HPRT1_exon3_1_Lb

    Plasmid

    #155053

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide3

    Plasmid

    #125430

    Purpose

    CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCR1319

    Plasmid

    #111116

    Purpose

    Expresses UBE2T_+_202311076.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    UBE2T gRNA (UBE2T Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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