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tetO-NKX2.5
(Plasmid #46029)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46029 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    tetO-Gateway
  • Total vector size (bp) 10000
  • Modifications to backbone
    NKX2.5 cloned into Gateway site of tetO-Gateway
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeo marker is outside the LTRs and will not be packaged into virus.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NKX2.5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1015
  • Entrez Gene
    NKX2-5 (a.k.a. CHNG5, CSX, CSX1, HLHS2, NKX2.5, NKX2E, NKX4-1, VSD3)
  • Promoter tetO
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • His (C terminal on backbone)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tetO-NKX2.5 was a gift from John Gearhart (Addgene plasmid # 46029 ; http://n2t.net/addgene:46029 ; RRID_Addgene_46029)
  • For your References section:

    Optimization of direct fibroblast reprogramming to cardiomyocytes using calcium activity as a functional measure of success. Addis RC, Ifkovits JL, Pinto F, Kellam LD, Esteso P, Rentschler S, Christoforou N, Epstein JA, Gearhart JD. J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 10.1016/j.yjmcc.2013.04.004. 10.1016/j.yjmcc.2013.04.004 PubMed 23591016