|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||46027||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 10565
Modifications to backboneGCaMP5-2A-Zeo cloned into TroponinT-Gateway using LR Recombinase.
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1800
- Promoter TNNT2
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Please note that Addgene's sequencing results identified nucleotide mismatches at bp# 3408, 4035, 4136 & 4641 when compared to the full plasmid sequence. According to the depositing laboratory, these differences are not a concern for the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:TroponinT-GCaMP5-Zeo was a gift from John Gearhart (Addgene plasmid # 46027 ; http://n2t.net/addgene:46027 ; RRID:Addgene_46027)
For your References section:Optimization of direct fibroblast reprogramming to cardiomyocytes using calcium activity as a functional measure of success. Addis RC, Ifkovits JL, Pinto F, Kellam LD, Esteso P, Rentschler S, Christoforou N, Epstein JA, Gearhart JD. J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 10.1016/j.yjmcc.2013.04.004. 10.1016/j.yjmcc.2013.04.004 PubMed 23591016