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Massively parallel reporter assays in cultured Mammalian cells.
Melnikov A, Zhang X, Rogov P, Wang L, Mikkelsen TS
J Vis Exp. 2014 Aug 17;(90). doi: 10.3791/51719.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
49349pMPRA1Basic backbone for transient transfection of Massively Parallel Reporter Assay (MPRA) libraries
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49350pMPRA2Backbone for transient transfection of Massively Parallel Reporter Assays (MPRA) libraries, pPGK-H2Kk for physical selection of transfected cells
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49351pMPRA3Backbone for transient transfection of Massively Parallel Reporter Assay (MPRA) libraries, pSV40-H2Kk for physical selection of transfected cells
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49352pMPRAdonor1Promoterless luc2 donor plasmid for MPRA library construction
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49353pMPRAdonor2minP-luc2 donor plasmid for MPRA library construction
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49354pMPRAdonor3pSV40mod-luc2 donor plasmid for MPRA library construction
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53538pMPRA1-CMV-CloverFluorescent reporter control for pMPRA1
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53539pMPRA2-CMV-CloverFluorescent reporter control for pMPRA2
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53540pMPRA3-CMV-CloverFluorescent reporter control for pMPRA3
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