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pMPRA2
(Plasmid #49350)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49350 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL4
  • Backbone manufacturer
    Promega
  • Backbone size (bp) 5862
  • Modifications to backbone
    Removed luc2 gene. Inserted cat-ccdB and PGK-H2Kk cassettes.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Truncated H2Kk cell surface receptor

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    Promega and Genscript (synthetic PGK-H2Kk insert)
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

Please note that the full plasmid sequence was assembled and may not be complete. The total size of the plasmid appears to be 7.5-8kb. This difference does not affect the function of the plasmid as described in the publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMPRA2 was a gift from Tarjei Mikkelsen (Addgene plasmid # 49350 ; http://n2t.net/addgene:49350 ; RRID:Addgene_49350)
  • For your References section:

    Massively parallel reporter assays in cultured Mammalian cells. Melnikov A, Zhang X, Rogov P, Wang L, Mikkelsen TS. J Vis Exp. 2014 Aug 17;(90). doi: 10.3791/51719. 10.3791/51719 PubMed 25177895