PurposeExpress self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51056||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4887
- Total vector size (bp) 5854
Modifications to backboneYeast ADH1 promoter and ADH1 terminator were cloned in between the BamHI and EcoRI site, and RGR-GFP gene was inserted inbetween the ADH1 promoter and terminator.
Vector typeBacterial Expression, Yeast Expression, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Insert Size (bp)211
- Promoter Yeast ADH1 promoter
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CCTCGTCATTGTTCTCGTTCC
- 3′ sequencing primer ACGTATCTACCAACGATTTGACC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRS316-RGR-GFP was a gift from Yunde Zhao (Addgene plasmid # 51056 ; http://n2t.net/addgene:51056 ; RRID:Addgene_51056)
For your References section:Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing. Gao Y, Zhao Y. J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152. 10.1111/jipb.12152 PubMed 24373158