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pcDNA3.1-Flag HBx
(Plasmid #42596)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42596 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Hepatitis B virus protein X
  • Alt name
    HBx
  • Species
    Hepatitis B virus
  • Insert Size (bp)
    492
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-Flag HBx was a gift from Ding Xue (Addgene plasmid # 42596 ; http://n2t.net/addgene:42596 ; RRID:Addgene_42596)
  • For your References section:

    Hepatitis B virus X protein targets Bcl-2 proteins to increase intracellular calcium, required for virus replication and cell death induction. Geng X, Huang C, Qin Y, McCombs JE, Yuan Q, Harry BL, Palmer AE, Xia NS, Xue D. Proc Natl Acad Sci U S A. 2012 Nov 6;109(45):18471-6. doi: 10.1073/pnas.1204668109. Epub 2012 Oct 22. 10.1073/pnas.1204668109 PubMed 23091012