pcDNA3.1-Flag HBx (G124L, I127A)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||42598||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert nameHepatitis B virus protein X (G124L, I127A)
Alt nameHBx (G124L, I127A)
Insert Size (bp)492
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer See 3' sequencing primer
- 3′ sequencing primer TAGAAGGCACAGTCGAGG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1-Flag HBx (G124L, I127A) was a gift from Ding Xue (Addgene plasmid # 42598 ; http://n2t.net/addgene:42598 ; RRID:Addgene_42598)
For your References section:Hepatitis B virus X protein targets Bcl-2 proteins to increase intracellular calcium, required for virus replication and cell death induction. Geng X, Huang C, Qin Y, McCombs JE, Yuan Q, Harry BL, Palmer AE, Xia NS, Xue D. Proc Natl Acad Sci U S A. 2012 Nov 6;109(45):18471-6. doi: 10.1073/pnas.1204668109. Epub 2012 Oct 22. 10.1073/pnas.1204668109 PubMed 23091012