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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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169,057 results

Showing: 6841 - 6860 of 169057 results

  1. TFORF1152

    Plasmid
    #142739
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MYOCD (MYOCD Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    142739-plasmid-map-sequence-id-462892

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  2. Perox-GA

    Plasmid
    #209868
    Purpose
    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP A
    Tags
    Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    209868-plasmid-map-sequence-id-415634

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  3. pGL3 basic EGFP

    Plasmid
    #128053
    Purpose
    EGFP reporter vector with restriction sites to clone in a promoter of interest, derived from pGL3 basic
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128053-plasmid-map-sequence-id-255087

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  4. pAAV-EF1a-C-CreintG

    Plasmid
    #69571
    Purpose
    One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cells
    Depositor
    Connie Cepko
    Article
    Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do
    Has Service
    AAV1
    Insert
    kozak consensus - C-CreintG
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    69571-plasmid-map-sequence-id-127747

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  6. pSLIK sh human Rb 1534 hyg

    Plasmid
    #31500
    Depositor
    Julien Sage
    Insert
    RB shRNA (RB1 Human)
    Use
    Lentiviral and RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    The target sequence for shRB 1534 is GAACGATTATCC…
    Available Since
    Aug. 18, 2011
    Availability
    Academic Institutions and Nonprofits only
    31500-plasmid-map-sequence-id-14427

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  8. pSBbi-GP

    Plasmid
    #60511
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60511-plasmid-map-sequence-id-222915

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  12. pcDNA5/FRT/TO-GAlphai1-RLuc8

    Plasmid
    #140973
    Purpose
    Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphai1-RLuc8 (GNAI1 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140973-plasmid-map-sequence-id-300655

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  13. POLG-pcDNA3.1

    Plasmid
    #191487
    Purpose
    Expresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.
    Depositor
    Hans Zempel
    Insert
    POLG (POLG Human)
    Tags
    V5 tag, 6X His
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    191487-plasmid-map-sequence-id-415364

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  14. AAV-mDlx-jGCaMP8s-WPRE (AAV9)

    Viral Prep
    #176755-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV-mDlx-jGCaMP8s-WPRE (#176755). In addition to the viral particles, you will also receive purified AAV-mDlx-jGCaMP8s-WPRE plasmid DNA. mDlx-driven expression of the calcium sensor jGCaMP8s in GABA-ergic interneurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    mDlx
    Available Since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only

  15. pAAV.CMV.ffLuciferase.SV40 (AAV8)

    Viral Prep
    #105532-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV.CMV.ffLuciferase.SV40 (#105532). In addition to the viral particles, you will also receive purified pAAV.CMV.ffLuciferase.SV40 plasmid DNA. CMV-driven ffLuciferase control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Tags
    ffLuciferase
    Available Since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  16. TFORF3550

    Plasmid
    #145026
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    mCherry
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    145026-plasmid-map-sequence-id-422981

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  18. pSupAR-MbPylRS(DiZPK)

    Plasmid
    #91705
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d
    Inserts
    pylS
    pylT
    Expression
    Bacterial
    Mutation
    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91705-plasmid-map-sequence-id-183278

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Showing: 6841 - 6860 of 169057 results