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181,758 results

Showing: 6841 - 6860 of 181758 results

  1. Luc-T20

    Plasmid
    #177941
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Publication
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Luc2 (ORF1ab )
    Expression
    Mammalian
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    177941-plasmid-map-sequence-id-348207

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  4. pIR-CMV-SCN1A-Variant-1-IRES-mScarlet

    Plasmid
    #162278
    Purpose
    Eukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
    Depositor
    Al George
    Publication
    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SCN1A Variant 1, stabilized (SCN1A Human)
    Tags
    IRES-mScarlet
    Expression
    Mammalian
    Mutation
    IVS intron inserted after bp2946. Silent codon c…
    Promoter
    CMV
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162278-plasmid-map-sequence-id-338003

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  5. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (AAV Retrograde)

    Viral prep
    #162378-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (#162378). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8m-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only

  7. pAAV-Ef1a-Con/Foff 2.0-sRGECO (AAV8)

    Viral prep
    #137127-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-sRGECO (#137127). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-sRGECO plasmid DNA. EF1a-driven, Cre-dependent expression of cytosolic sRGECO calcium sensor (inhibited in presence of Flp). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Available since
    May 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  9. pAAV-Ef1a-Coff/Fon-mCherry (AAV8)

    Viral prep
    #137134-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-mCherry (#137134). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-mCherry plasmid DNA. EF1a-driven, Flp-dependent expression of mCherry (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    mCherry (Flp-dependent)
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only

  10. pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE

    Plasmid
    #162380
    Purpose
    AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Has service
    AAV1, AAV9, and AAV Retrograde
    Insert
    jGCaMP8s
    Use
    AAV and Cre/Lox
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    S26M F286Y Q315H
    Promoter
    CAG
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162380-plasmid-map-sequence-id-318379

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  11. RED

    Plasmid
    #139835
    Purpose
    Expresses RNase H1-EGFP- dCas9 fusion protein in human cells
    Depositor
    Karim Mekhail
    Publication
    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Expression
    Mammalian
    Mutation
    dCas9 protein contains two mutations: D10A and H8…
    Promoter
    tet-inducible CMV
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139835-plasmid-map-sequence-id-310615

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  12. pLenti-PalmGRET

    Plasmid
    #158221
    Purpose
    BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.
    Depositor
    Charles P. Lai
    Publication
    Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014
    Insert
    PalmGFP-Nluc
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    158221-plasmid-map-sequence-id-309022

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  16. SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)

    Plasmid
    #149276
    Purpose
    Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs
    Depositor
    Savithramma Dinesh-Kumar
    Publication
    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
    Use
    T-dna vector
    Mutation
    T2318C (DNA) in PEBV coat protein sequence
    Available since
    June 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    149276-plasmid-map-sequence-id-300510

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  17. pDONR223 SARS-CoV-2 N

    Plasmid
    #149330
    Purpose
    Gateway-compatible Entry vector, with insert of the nucleocapsid (N) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Publication
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    N (N SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway Cloning
    Mutation
    Many synonymous changes due to codon optimization
    Available since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149330-plasmid-map-sequence-id-296491

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  18. pcDNA3-sACE2(WT)-sfGFP

    Plasmid
    #145171
    Purpose
    Mammalian expression plasmid for soluble ACE2-super folder GFP fusion
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145171-plasmid-map-sequence-id-292613

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  20. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

    Plasmid
    #113865
    Purpose
    Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.
    Depositor
    Manfred Ogris
    Publication
    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d
    Inserts
    CMV-eGFP
    TOP-iRFP
    PTCH1-mTurquoise2
    CBF-tdTomato
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113865-plasmid-map-sequence-id-220242

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Showing: 6841 - 6860 of 181758 results