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Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle.
Lo HP, Lim YW, Xiong Z, Martel N, Ferguson C, Ariotti N, Giacomotto J, Rae J, Floetenmeyer M, Moradi SV, Gao Y, Tillu VA, Xia D, Wang H, Rahnama S, Nixon SJ, Bastiani M, Day RD, Smith KA, Palpant NJ, Johnston WA, Alexandrov K, Collins BM, Hall TE, Parton RG
J Cell Biol. 2021 Dec 6;220(12). pii: 212693. doi: 10.1083/jcb.201905065. Epub 2021 Oct 11.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
126563actc1b-GcAMP5Muscle specific expression of the calcium reporter GcAMP5 in zebrafish. Parton lab clone APT
126566Cavin4b in pBluescript(SK+)For generating RNA probes for in situ hybridisation. Parton lab clone IRZ
126567Cavin4a in pBluescript(SK+)For generating RNA probes for in situ hybridisation. Parton lab clone ISB
126569pME-GcAMP5Multisite gateway middle entry clone containing zebrafish calcium reporter GcAMP5. Parton lab clone ANR.
126572p3E-CloverMultisite gateway vector for 3' tagging with Clover. Parton lab clone DJZ
126574p3E-BIN1_tv8Multisite gateway middle entry vector containing human BIN1 transcriptional variant 8 (muscle specific) with no ATG. Parton lab clone DVA.
176007Cavin4-mCherryMammalian expression of Mouse Cavin4-mCherry. Parton lab clone JSG
176008Cavin4b-mCherryMammalian expression of zebrafish Cavin4b-mCherry. Parton lab clone HYO
176009Cavin4b(PRDmt)-mCherryMammalian expression of zebrafish Cavin4b(PRDmt)-mCherry. Parton lab clone JMD
176010Cavin4a-mCherryMammalian expression of zebrafish Cavin4a-mCherry. Parton lab clone HYM
176011Cavin4b-EGFPMammalian expression of zebrafish Cavin4b-EGFP. Parton lab clone HXM
176012EGFP-HsBIN1_tv8Mammalian expression of Human EGFP-Bin1 (transcriptional variant 8, muscle specific). Parton lab clone HBH
176013EGFP-Bin1aMammalian expression of zebrafish EGFP-Bin1a. Parton lab clone HYA
176014EGFP-Bin1bMammalian expression of zebrafish EGFP-Bin1b. Parton lab clone HYG
176015EGFPMammalian expression of EGFP. Parton lab clone DFS
176016mCherryMammalian expression of mCherry. Parton lab clone DFQ
176017pME-Cavin4b_PRDmtMultisite gateway middle entry clone containing zebrafish Cavin4b_PRDmt with no stop codon. Parton lab clone JLY.
176018SITS-Cavin4a-EGFPLeishmania cell free expression of zebrafish Cavin4a-EGFP. Parton lab clone GRK
176019SITS-Cavin4b-EGFPLeishmania cell free expression of zebrafish Cavin4b-EGFP. Parton lab clone GRJ
176020SITS-EGFP-Bin1aLeishmania cell free expression of zebrafish EGFP-Bin1a. Parton lab clone GRQ
176021SITS-EGFP-Bin1bLeishmania cell free expression of zebrafish EGFP-Bin1b. Parton lab clone GRS
176022SITS-Cavin4a-mCherryLeishmania cell free expression of zebrafish Cavin4a-mCherry. Parton lab clone GWU
176023SITS-Cavin4b-mCherryLeishmania cell free expression of zebrafish Cavin4b-mCherry. Parton lab clone GWR
176024SITS-mCherry-Bin1aLeishmania cell free expression of zebrafish mCherry-Bin1a. Parton lab clone GSS
176025SITS-mCherry-Bin1bLeishmania cell free expression of zebrafish mCherry-Bin1b. Parton lab clone GSU

Antibodies from Article