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Luminescent and fluorescent triple reporter plasmid constructs for Wnt, Hedgehog and Notch pathway.
Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M
PLoS One. 2019 Dec 20;14(12):e0226570. doi: 10.1371/journal.pone.0226570. eCollection 2019.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
113708pMuLE_ENTR_CBF-tdTomato_L3-L2Single pathway reporter; notch promoter-driven expression of tdTomato; MultiSite Gateway entry vector.
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113712pMuLE_ENTR_12GLI-FLuc_R4-R3Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.
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113715EF.hICN1Constitutive expression of hICN1, e.g. for hedgehog-induction. Based on addgene #17623, with CMV.GFP removed.
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113862pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLucTriple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.
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113864pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3Single pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.
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113865pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomatoTriple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.
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113866pMuLE_ENTR_TOP-iRFP_L5-L4Single pathway reporter; wnt promoter-driven expression of iRFP; MultiSite Gateway entry vector.
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113867pMuLE_ENTR_TOP-NLuc1.1_L5-L4Single pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.
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113868pMuLE_ENTR_CBF-GLuc_L3-L2Single pathway reporter; notch promoter-driven expression of secreted Gaussia luciferase; MultiSite Gateway entry vector.
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Recombinant Antibodies from Article