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pMuLE_ENTR_CBF-tdTomato_L3-L2
(Plasmid #113708)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113708 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMuLE ENTR SV40 tdTomato L3-L2
  • Backbone size w/o insert (bp) 4403
  • Total vector size (bp) 4515
  • Vector type
    Mammalian Expression ; MuLE Gateway Entry Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CBF (notch dependent promoter)
  • Alt name
    CBFRE
  • Insert Size (bp)
    446

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    CBF:H2B-Venus was a gift from Anna-Katerina Hadjantonakis (Addgene plasmid # 44211). Backbone is included in the MuLE kit, which was a gift from Ian Frew (Addgene kit #1000000060).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMuLE_ENTR_CBF-tdTomato_L3-L2 was a gift from Manfred Ogris (Addgene plasmid # 113708 ; http://n2t.net/addgene:113708 ; RRID:Addgene_113708)
  • For your References section:

    Luminescent and fluorescent triple reporter plasmid constructs for Wnt, Hedgehog and Notch pathway. Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M. PLoS One. 2019 Dec 20;14(12):e0226570. doi: 10.1371/journal.pone.0226570. eCollection 2019. 10.1371/journal.pone.0226570 PubMed 31860685