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pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3
(Plasmid #113864)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113864 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMuLE ENTR MCS R4-R3
  • Backbone size w/o insert (bp) 2775
  • Total vector size (bp) 4959
  • Vector type
    Mammalian Expression ; MuLE Gateway Entry Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    PTCH1 (hedgehog dependent promoter)
  • Alt name
    hPtch1 prom wt
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1375

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    mTurquoise2
  • Insert Size (bp)
    817

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pGL3b hPtch1 prom wt was a gift from Fritz Aberger. pmTurquoise2-NES was a gift from Dorus Gadella (Addgene plasmid#36206). Backbone is included in the MuLE kit, which was a gift from Ian Frew (Addgene kit #1000000060).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Winklmayr M, Schmid C, Laner-Plamberger S, Kaser A, Aberger F, Eichberger T, et al. Non-consensus GLI binding sites in Hedgehog target gene regulation. BMC molecular biology. 2010;11(1):2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3 was a gift from Manfred Ogris (Addgene plasmid # 113864 ; http://n2t.net/addgene:113864 ; RRID:Addgene_113864)
  • For your References section:

    Luminescent and fluorescent triple reporter plasmid constructs for Wnt, Hedgehog and Notch pathway. Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M. PLoS One. 2019 Dec 20;14(12):e0226570. doi: 10.1371/journal.pone.0226570. eCollection 2019. 10.1371/journal.pone.0226570 PubMed 31860685