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EF.hICN1
(Plasmid #113715)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113715 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    EF.hICN1.CMV.GFP
  • Total vector size (bp) 9166
  • Modifications to backbone
    Removed CMV.GFP
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    EF.hICN1.CMV.GFP was a gift from Linzhao Cheng (Addgene plasmid #17623).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The hICN1 insert contains a difference that results in A184S compared to the NCBI reference. This difference does not impact the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EF.hICN1 was a gift from Manfred Ogris (Addgene plasmid # 113715 ; http://n2t.net/addgene:113715 ; RRID:Addgene_113715)
  • For your References section:

    Luminescent and fluorescent triple reporter plasmid constructs for Wnt, Hedgehog and Notch pathway. Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M. PLoS One. 2019 Dec 20;14(12):e0226570. doi: 10.1371/journal.pone.0226570. eCollection 2019. 10.1371/journal.pone.0226570 PubMed 31860685