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PurposeGli protein-dependent EGFP reporter for analysis of Hedgehog signaling
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Depositing Lab
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Depositing OrganizationStanford University
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84602 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 84602-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 84602-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL3-Basic
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3900
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameeGFP
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Speciesjellyfish
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Insert Size (bp)714
- Promoter 8X Gli-binding site
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site HpaI (not destroyed)
- 5′ sequencing primer EBV-rev
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPlasmid derived from pGl3-B-8XGliBS:Luc (Sasaki, et al., Development, 1997)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid generated by replacing the firefly luciferase cDNA in the 8XGliBS:Luc vector (Sasaki, et al, 1997) with EGFP cDNA from the pEGFP-C1 vector (Clontech).
DNA (Catalog # 84602-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 84602-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3b-8XGliBS:EGFP was a gift from James Chen (Addgene plasmid # 84602 ; http://n2t.net/addgene:84602 ; RRID:Addgene_84602) -
For your References section:
Small-molecule inhibitors reveal multiple strategies for Hedgehog pathway blockade. Hyman JM, Firestone AJ, Heine VM, Zhao Y, Ocasio CA, Han K, Sun M, Rack PG, Sinha S, Wu JJ, Solow-Cordero DE, Jiang J, Rowitch DH, Chen JK. Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):14132-7. doi: 10.1073/pnas.0907134106. Epub 2009 Aug 5. 10.1073/pnas.0907134106 PubMed 19666565
Map uploaded by the depositor.
