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pMuLE_ENTR_12GLI-FLuc_R4-R3
(Plasmid #113712)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113712 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMuLE ENTR MCS R4-R3
  • Backbone size w/o insert (bp) 2775
  • Total vector size (bp) 6801
  • Vector type
    Mammalian Expression ; MuLE Gateway Entry Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    12GLI-FLuc
  • Insert Size (bp)
    4034

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer M13
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    12GLI-RETKO-luc was a gift from Peter Zaphiropoulos. Backbone is included in the MuLE kit, which was a gift from Ian Frew (Addgene kit #1000000060).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Citation for 12GLI-RETKO-luc: Kogerman P, Grimm T, Kogerman L, Krause D, Undén AB, Sandstedt B, et al. Mammalian suppressor-of-fused modulates nuclear–cytoplasmic shuttling of Gli-1. Nature cell biology. 1999;1(5):312.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMuLE_ENTR_12GLI-FLuc_R4-R3 was a gift from Manfred Ogris (Addgene plasmid # 113712 ; http://n2t.net/addgene:113712 ; RRID:Addgene_113712)
  • For your References section:

    Luminescent and fluorescent triple reporter plasmid constructs for Wnt, Hedgehog and Notch pathway. Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M. PLoS One. 2019 Dec 20;14(12):e0226570. doi: 10.1371/journal.pone.0226570. eCollection 2019. 10.1371/journal.pone.0226570 PubMed 31860685