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pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc
(Plasmid #113862)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 113862 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMuLE Lenti Dest Neo
  • Backbone size w/o insert (bp) 9237
  • Total vector size (bp) 15489
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418) ; eGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CMV-eGFP
  • Insert Size (bp)
    1475

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    TOP-NLuc1.1
  • Alt name
    M50 Super 8x TOPFlash, promoter only
  • Alt name
    NL1.1
  • Insert Size (bp)
    822

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    12GLI-Fluc
  • Alt name
    firefly luciferase
  • Insert Size (bp)
    4136

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    CBF-GLuc
  • Alt name
    CBFRE
  • Alt name
    Gaussia luciferase
  • Insert Size (bp)
    1090

Cloning Information for Gene/Insert 4

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Backbone is included in the MuLE kit, which was a gift from Ian Frew (Addgene kit #1000000060).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc was a gift from Manfred Ogris (Addgene plasmid # 113862 ; http://n2t.net/addgene:113862 ; RRID:Addgene_113862)
  • For your References section:

    Luminescent and fluorescent triple reporter plasmid constructs for Wnt, Hedgehog and Notch pathway. Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M. PLoS One. 2019 Dec 20;14(12):e0226570. doi: 10.1371/journal.pone.0226570. eCollection 2019. 10.1371/journal.pone.0226570 PubMed 31860685