pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc
(Plasmid #113862)

• Purpose: Triple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.
• Depositing Lab: Manfred Ogris
• Publication: Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. doi: 10.1371/journal.pone.0226570. eCollection 2019.

Backbone

• Vector backbone: pMuLE Lenti Dest Neo
• Backbone size w/o insert (bp): 9237
• Total vector size (bp): 15489
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418) ; eGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: CMV-eGFP
• Insert Size (bp): 1475

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 rev

Gene/Insert 2

• Gene/Insert name: TOP-NLuc1.1
• Alt name: M50 Super 8x TOPFlash, promoter only
• Alt name: NL1.1
• Insert Size (bp): 822

Cloning Information for Gene/Insert 2

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 rev

Gene/Insert 3

• Gene/Insert name: 12GLI-Fluc
• Alt name: firefly luciferase
• Insert Size (bp): 4136

Cloning Information for Gene/Insert 3

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 rev

Gene/Insert 4

• Gene/Insert name: CBF-GLuc
• Alt name: CBFRE
• Alt name: Gaussia luciferase
• Insert Size (bp): 1090

Cloning Information for Gene/Insert 4

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13 rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Backbone is included in the MuLE kit, which was a gift from Ian Frew (Addgene kit #1000000060).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry