pNeae2
(Plasmid #168300)

• Purpose: Bacterial surface display of polypeptides (nanobodies) in E. coli
• Depositing Lab: Luis Ángel Fernández
• Publication: Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi: 10.1371/journal.pone.0075126. eCollection 2013.

Backbone

• Vector backbone: pAK-Not
• Backbone manufacturer: Luis Angel Fernández
• Backbone size w/o insert (bp): 3735
• Total vector size (bp): 5832
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
• Species: Synthetic
• Insert Size (bp): 2097
• Promoter: LacI-Plac
• Tags / Fusion Proteins:
  • E-tag (C terminal on insert)
  • 6xHis (C terminal on insert)
  • myc-tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: N/A (unknown if destroyed)
• 3′ cloning site: N/A (unknown if destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pNeae2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry