pATLIC
(Plasmid #110287)

• Purpose: A backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)
• Depositing Lab: In-Geol Choi
• Publication: Ko et al Appl Environ Microbiol. 2012 May;78(9):3051-8. doi: 10.1128/AEM.07004-11. Epub 2012 Feb 17.

Backbone

• Vector backbone: pBAD
• Backbone size w/o insert (bp): 3986
• Total vector size (bp): 5519
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: yfaL
• Alt name: autotransporter
• Species: E. coli
• Insert Size (bp): 1533
• GenBank ID: AAA74094
• Promoter: araBAD promoter

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: GAAGGAGATATAAGGATGCGGATTATCTTTCTACGCAAG
• 3′ sequencing primer: ATGATGGTGATGGTGCCATTTCACCGTCATCGACAAA

Resource Information

• Supplemental Documents:
  • patlic.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene QC finds S92G, G99S, and K330R mutations in yfaL as well as a stop codon inserted before the His tag. The depositor notes these are not of functional concern