pHEA
(Plasmid #168297)

• Purpose: Bacterial surface display of polypeptides (nanobodies) in E. coli
• Depositing Lab: Luis Ángel Fernández
• Publication: Marin et al J Bacteriol. 2010 Nov;192(21):5588-602. doi: 10.1128/JB.00432-10. Epub 2010 Aug 27.

Backbone

• Vector backbone: pAK-Not
• Backbone manufacturer: Luis Angel Fernández
• Backbone size w/o insert (bp): 3735
• Total vector size (bp): 4974
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
• Species: Escherichia coli
• Insert Size (bp): 1239
• Promoter: LacI-Plac
• Tags / Fusion Proteins:
  • PelB signal peptide (N terminal on insert)
  • 6xHis (N terminal on insert)
  • E-tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: N/A (unknown if destroyed)
• 3′ cloning site: N/A (unknown if destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pHEA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry