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(Plasmid #168297)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168297 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
    Luis Angel Fernández
  • Backbone size w/o insert (bp) 3735
  • Total vector size (bp) 4974
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
  • Species
    Escherichia coli
  • Insert Size (bp)
  • Promoter LacI-Plac
  • Tags / Fusion Proteins
    • PelB signal peptide (N terminal on insert)
    • 6xHis (N terminal on insert)
    • E-tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site N/A (unknown if destroyed)
  • 3′ cloning site N/A (unknown if destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHEA was a gift from Luis Ángel Fernández (Addgene plasmid # 168297 ; ; RRID:Addgene_168297)
  • For your References section:

    Comparative analysis of the biochemical and functional properties of C-terminal domains of autotransporters. Marin E, Bodelon G, Fernandez LA. J Bacteriol. 2010 Nov;192(21):5588-602. doi: 10.1128/JB.00432-10. Epub 2010 Aug 27. 10.1128/JB.00432-10 PubMed 20802036