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(Plasmid #101156)


Item Catalog # Description Quantity Price (USD)
Plasmid 101156 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    Promega L482A
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2678
  • Total vector size (bp) 4331
  • Modifications to backbone
    Sequence modified from T7 promoter to ATG of the luciferase AGACCCAAGCTTTCAGATCCGCTAGCGCTACCGGGGATCCagccacc
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Firefly luciferase
  • Alt name
  • Species
    Photinus pyralis
  • Insert Size (bp)
  • Promoter T7 promoter

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer AATACGCAAACCGCCTCTC
  • 3′ sequencing primer GGTGATGTCGGCGATATAGG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is only for the production of transcript not for expression in mammalian cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T7-CMVtrans-FFLuc-polyA was a gift from Marcel Bruchez (Addgene plasmid # 101156 ; ; RRID:Addgene_101156)
  • For your References section:

    In Vitro Reversible Translation Control Using gammaPNA Probes. Canady TD, Telmer CA, Oyaghire SN, Armitage BA, Bruchez MP. J Am Chem Soc. 2015 Aug 19;137(32):10268-75. doi: 10.1021/jacs.5b05351. Epub 2015 Aug 4. 10.1021/jacs.5b05351 PubMed 26241615