232,493 results

1. pLv-EF1a-Parkin-A92mKO2

   Plasmid

   #213559

   Purpose

   Lentiviral vector for stable expression of Parkin, internally tagged with mKO2

   Depositor

   Steven Altschuler, Lani Wu

   Article

   Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

   Insert

   Parkin (PRKN Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV-EF1amCherry-W

   Plasmid

   #159295

   Purpose

   Lentiviral vector expressing mCherry driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   human EF1a promoter

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUBC-popTag-QtE-mScarlet

   Plasmid

   #240101

   Purpose

   Mammalian lentiviral vector for expressing the mScarlet-tagged 50nm-GEMs, encoded by the encapsulin protein from Quasibacillus thermotolerans and fused to a PopZ tag.

   Depositor

   Liam Holt

   Article

   Xie et al Mol Cell. 2024 Jul 25;84(14):2698-2716.e

   Insert

   Encapsulin
   

   Use

   Lentiviral

   Tags

   mScarlet and popZ

   Expression

   Bacterial and Mammalian

   Promoter

   UBC promoter

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. SepRS(2)/pSertRNA(B4)/EF-Sep

   Plasmid

   #173897

   Purpose

   The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.

   Depositor

   Jason W Chin

   Article

   Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d

   Inserts

   SepRS(2)
   pSertRNA
   EF-Sep
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMJ915

   Plasmid

   #69090

   Purpose

   Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

   Depositor

   Jennifer Doudna

   Article

   Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

   Insert

   cas9
   

   Tags

   MBP, hexa His, and two SV40 NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMJ915

   Plasmid

   #69090

   Purpose

   Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

   Depositor

   Jennifer Doudna

   Article

   Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

   Insert

   cas9
   

   Tags

   MBP, hexa His, and two SV40 NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. W45A mutant of H3K9me3 biosensor

   Plasmid

   #120808

   Purpose

   FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
   

   Expression

   Mammalian

   Mutation

   Tryptophan 45 is mutated to Alanine 45 on HP1 dom…

   Promoter

   CMV

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-11-LECT2-I40

   Plasmid

   #183032

   Purpose

   Expression of LECT2 I40 variant protein from T7 promoter

   Depositor

   Gareth Morgan

   Article

   Belonogov et al bioRxiv 2022.03.01.482540

   Insert

   LECT2 (LECT2 Human)
   

   Expression

   Bacterial

   Mutation

   I40 variant

   Promoter

   T7

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. plentiCMV-sct-pp65-b2m-HLA-A0201

   Plasmid

   #196511

   Purpose

   Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   single chain of a pp65 peptide, b2m and HLA A0201
   

   Use

   Lentiviral

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Y84C

   Promoter

   CMV

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. GalT-RpHLuorin2

    Plasmid

    #171719

    Purpose

    Trans-Golgi expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)
    

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJ609 Flag-Sumo1

    Plasmid

    #49046

    Purpose

    Mammalian expression of N-terminally tagged Sumo1

    Depositor

    Lianna Swanson

    Insert

    SUMO1 (SUMO1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJ609 Flag-Sumo1

    Plasmid

    #49046

    Purpose

    Mammalian expression of N-terminally tagged Sumo1

    Depositor

    Lianna Swanson

    Insert

    SUMO1 (SUMO1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-Flpo

    Plasmid

    #60662

    Purpose

    Expresses pCAG-Flpo; codon-optimized for mammalian expression

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    Flpo
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-Flpo

    Plasmid

    #60662

    Purpose

    Expresses pCAG-Flpo; codon-optimized for mammalian expression

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    Flpo
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)/AUG-nLuc-3XFLAG

    Plasmid

    #127299

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3 promoter EGFP

    Plasmid

    #128054

    Purpose

    EGFP reporter vector with restriction sites to clone in an enhancer of interest, derived from pGL3 promoter

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-6xHis-(pos15)GFP

    Plasmid

    #89248

    Purpose

    [Edit] Expresses GFP with +15 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos15)-GFP
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-6xHis-(pos15)GFP

    Plasmid

    #89248

    Purpose

    [Edit] Expresses GFP with +15 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos15)-GFP
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFR WT

    Plasmid

    #11011

    Purpose

    Retroviral construct for expressing human EGFR in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFR WT

    Plasmid

    #11011

    Purpose

    Retroviral construct for expressing human EGFR in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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