168,499 results

1. MDM2p-Mdm2-YFP

   Plasmid

   #53962

   Purpose

   Expresses YFP tagged Mdm2 from MDM2 promoter

   Depositor

   Uri Alon, Galit Lahav

   Article

   Lahav et al Nat Genet. 2004 Feb;36(2):147-50. Epub 2

   Insert

   MDM2 (MDM2 Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   MDM2

   Available Since

   Aug. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-IRES-GFP-human-Caspase-4

   Plasmid

   #183356

   Purpose

   Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-4 (CASP4 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Promoter

   MESV

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKH3-TRIM28

   Plasmid

   #45569

   Depositor

   Fanxiu Zhu

   Article

   Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

   Insert

   TRIM28 (TRIM28 Human)
   

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 29, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. PBCAG-eGFP

   Plasmid

   #40973

   Depositor

   Joseph Loturco

   Article

   Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

   Inserts

   PiggyBac transposon 3' terminal repeats
   PiggyBac 5' terminal repeats
   

   Expression

   Mammalian

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213029

   Purpose

   Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
   

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. MXS Chaining Kit

   Plasmid Kit

   #1000000064

   Purpose

   MXS-chaining system for assembly of constructs optimized for synthetic biology applications in mammalian systems. 14 different fluorescent proteins, 10 mammalian promoters/enhancers, 3 polyA signals

   Depositor

   Pierre Neveu

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

7. V5-miniTurbo-NES_pCDNA3

   Plasmid

   #107170

   Purpose

   expresses V5-tagged miniTurbo in the mammalian cytosol

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Expression

   Mammalian

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 (AAV5)

   Viral Prep

   #100835-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 (#100835). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Promoter

   CAG

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya

   Plasmid

   #186980

   Purpose

   mammalian expression of Cluc-Gluc reporter

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   Cluc, Gluc
   

   Use

   Luciferase

   Available Since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRK892

    Plasmid

    #71803

    Purpose

    Expression of mutated L. gasseri gusA from strong Ppgm promoter

    Depositor

    Rodolphe Barrangou, Todd Klaenhammer

    Article

    Duong et al Microb Biotechnol. 2011 May;4(3):357-67.

    Insert

    gusA3
    

    Expression

    Bacterial

    Mutation

    D573A

    Promoter

    Ppgm

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHrD-IRES-Fluc

    Plasmid

    #194250

    Purpose

    Human RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!

    Depositor

    Samson Jacob

    Article

    McCool et al RNA. 2022 Nov 2. pii: rna.079149.122. do

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Promoter

    human rDNA promoter and beginning of 5' ETS …

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOS011: superecliptic pHluorin (mitochondrial)

    Plasmid

    #163050

    Purpose

    superecliptic pHluorin (mitochondrial)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    superecliptic pHluorin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLIK CA ROCK2

    Plasmid

    #84649

    Purpose

    Lentiviral expression of doxycycline-inducible constitutively active ROCK2 and co-expression of Venus

    Depositor

    Sanjay Kumar

    Article

    Wong et al Cancer Res. 2015 Mar 15;75(6):1113-22. d

    Insert

    ROCK2 (ROCK2 Bovine)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Constitutive Active (please see depositor comment…

    Available Since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. piggyBAC-H2B mCherry-XL (R125K)

    Plasmid

    #189926

    Purpose

    Expresses nuclear-localized mCherry-XL (R125K) in mammalian cells

    Depositor

    Ralph Jimenez

    Article

    Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

    Insert

    mCherry-XL (R125K)
    

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    differs from mCherry by R125K, W143S, I161V, Q163…

    Promoter

    CMV7

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pG106 - KA

    Plasmid

    #178041

    Purpose

    Bacteroides - Escherichia coli shuttle vector with ErmF and ErmAM, Kan and Amp selection markers

    Depositor

    Janina Lewis

    Article

    Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

    Type

    Empty backbone

    Use

    Shuttle vector

    Expression

    Bacterial

    Available Since

    Feb. 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pMpGWB104

    Plasmid

    #68558

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    GUS

    Expression

    Plant

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF0174

    Plasmid

    #141541

    Purpose

    Lentiviral vector for overexpressing the RELA transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RELA (RELA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. SPLICS Mt-ER- PM Long P2A

    Plasmid

    #164109

    Purpose

    Detect the long Plasma membrane--mitochondria-Endoplasmic reticulum contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP and YFP PM-Mt-ER Long P2A
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHH0103_YWHAG_14-3-3

    Plasmid

    #109905

    Purpose

    Protein expression and purification of YWHAG_14-3-3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    YWHAG_14-3-3 (YWHAG Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. MI-Luciferase-IRES-mCherry

    Plasmid

    #75020

    Purpose

    Encodes the expression of Luciferase and mCherry

    Depositor

    Xiaoping Sun

    Article

    Mallampati et al Blood. 2014 Jun 26;123(26):4064-76. doi:

    Inserts

    firefly Luciferase
    mCherry
    

    Use

    Luciferase and Retroviral

    Available Since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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