168,499 results

1. pMSCV-dCasRx-METTL3-PURO

   Plasmid

   #174218

   Purpose

   Expresses dCasRx-METTL3 in mammalian cell

   Depositor

   Qi Xie

   Article

   Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

   Insert

   dCasRx-METTL3
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   pMSCV

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-Lyn11-FRB

   Plasmid

   #155228

   Purpose

   membrane anchor for FKBP-FRB system

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   Membrane targeted (through Lyn11) FRB for use with FKBP-FRB dimerization system
   

   Expression

   Mammalian

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-DIO-GRAB-rDA3m

   Plasmid

   #208708

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA3m
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-EGFP-C1-HsXRN1_K

   Plasmid

   #146784

   Purpose

   Mammalian Expression of HsXRN1

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   HsXRN1 (XRN1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-EF1

   Plasmid

   #72266

   Purpose

   Express gene of interest under EF-1 promoter

   Depositor

   Kazuhiro Oka

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 11, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. lenti-sgRNA hygro

   Plasmid

   #104991

   Purpose

   This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPRa_all-in-one

   Plasmid

   #183695

   Purpose

   Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p70a-T7RNAP

   Plasmid

   #105683

   Purpose

   Expresses T7 polymerase in cell-free TXTL.

   Depositor

   Chase Beisel

   Article

   Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

   Insert

   T7 polymerase
   

   Expression

   Bacterial

   Promoter

   OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWM_12x601_35bpLinker

   Plasmid

   #157790

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_35bp linker
   

   Expression

   Bacterial

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-IRES-NUP98::NSD1-GFP

    Plasmid

    #236201

    Purpose

    Expresses NUP98::NSD1 fusion protein in mammalian cells

    Depositor

    Michael Heuser

    Article

    Mohanty et al Blood Adv. 2025 May 7:bloodadvances.2024

    Insert

    NUP98::NSD1 (NUP98, NSD1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZac2.1 gfaABC1D-cyto-GCaMP8f

    Plasmid

    #186050

    Purpose

    AAV-mediated expression of GCaMP8f under the control of GfaABC1D promoter

    Depositor

    Loren Looger

    Insert

    jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    GfaABC1D

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. VAChT-pHuji

    Plasmid

    #105319

    Purpose

    VAChT tagged with pH-sensitive red fluorescent protein pHuji

    Depositor

    Justin Taraska

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    VAChT (Slc18a3 Rat)
    

    Tags

    pHuji

    Expression

    Mammalian

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF2465

    Plasmid

    #142946

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    IRF6 (IRF6 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. TfR-pHuji

    Plasmid

    #61505

    Purpose

    Human transferrin receptor tagged with pH sensitive red fluorescent protein pHuji

    Depositor

    David Perrais

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    Transferrin receptor (TFRC Human)
    

    Tags

    pHuji

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. RUFY1-GFP

    Plasmid

    #197394

    Purpose

    Expresses RUFY1 in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Keren-Kaplan et al Nat Commun. 2022 Mar 21;13(1):1506. doi:

    Insert

    RUFY1 (RUFY1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. SuperClomeleon

    Plasmid

    #203724

    Purpose

    An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride

    Depositor

    George Augustine

    Article

    Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.

    Insert

    Cerulean linked topaz fluorescent protein
    

    Expression

    Bacterial and Mammalian

    Mutation

    Cerulean, modified Topaz Fluorescent Protein with…

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.CAG.GCaMP6s.WPRE.SV40

    Plasmid

    #100844

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

    Promoter

    CAG

    Available Since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC016 - LwCas13a guide expression backbone with U6 promoter

    Plasmid

    #91906

    Purpose

    Backbone for cloning LwCas13a guides under U6 promoter

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Has Service

    Cloning Grade DNA

    Insert

    LwCas13a Guide site
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAS9id

    Plasmid

    #141251

    Purpose

    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-EF1aL-WT_ABCA3-GFP

    Plasmid

    #188548

    Purpose

    Lentiviral vector allowing for EF1aL-driven constitutive expression of wildtype human ABCA3:GFP fusion protein

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    ABCA3:GFP (ABCA3 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Promoter

    EF1aL

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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