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Hsp68-CreER(TM) (DM#264)
(Plasmid #15028)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15028 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    modified pBluescript II KS
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mammalian Expression, Cre/Lox ; for making transgenic constructs

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cre-ER(TM)
  • Alt name
    Cre-ER (TM)
  • Insert Size (bp)
    3700
  • Tags / Fusion Proteins
    • Hsp68 promoter (N terminal on insert)
    • polyA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site FseI (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cre-ER(TM) was a gift from Andy McMahon. Hsp68 was a gift from Maureen Gannon.
  • Terms and Licenses

Depositor Comments

The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision.

See Author's Map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Hsp68-CreER(TM) (DM#264) was a gift from Douglas Melton (Addgene plasmid # 15028 ; http://n2t.net/addgene:15028 ; RRID:Addgene_15028)