|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14998||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerBD Biosciences
- Backbone size w/o insert (bp) 5300
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)3000
Entrez GeneMertk (a.k.a. Eyk, Me, Mer, Ny, Nyk, nmf1, nmf12)
/ Fusion Protein
- IRES EGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The full-length mouse Mer (994 amino acids) was cloned from a 16-day-old mouse embryo cDNA library. Addgene's quality control found the closest match to the insert sequence is mouse Mer (AA17-994) Sequence ID: NP_032613.1, with mutations I516V and Q860R. The plasmid should function as described in the associated article listed on the plasmid page above.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pIRES2-EGFP Mer was a gift from Raymond Birge (Addgene plasmid # 14998 ; http://n2t.net/addgene:14998 ; RRID:Addgene_14998)
For your References section:A role for Mer tyrosine kinase in alphavbeta5 integrin-mediated phagocytosis of apoptotic cells. Wu Y, Singh S, Georgescu MM, Birge RB. J Cell Sci. 2005 Feb 1. 118(Pt 3):539-53. 10.1242/jcs.01632 PubMed 15673687