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Ubc-megf10-GFP
(Plasmid #40207)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40207 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 40207-D50 50 μg of DNA in Tris buffer $495
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DNA 40207-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pUb-GFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Megf10
  • Alt name
    multiple EGF-like-domains 10
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3400
  • GenBank ID
    NM_001001979.2
  • Entrez Gene
    Megf10 (a.k.a. 3000002B06Rik, Gm331)
  • Tag / Fusion Protein
    • GFP fusion (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ubc-megf10-GFP was a gift from Joshua Sanes (Addgene plasmid # 40207 ; http://n2t.net/addgene:40207 ; RRID:Addgene_40207)

  • For your References section:

    MEGF10 and MEGF11 mediate homotypic interactions required for mosaic spacing of retinal neurons. Kay JN, Chu MW, Sanes JR. Nature. 2012 Mar 11;483(7390):465-9. doi: 10.1038/nature10877. 10.1038/nature10877 PubMed 22407321
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