Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||29452||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3900
Vector typefor ISH
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt namecollapsin 2
Alt namesemaphorin 3D
SpeciesM. musculus (mouse)
Insert Size (bp)6000
Mutationinsert is not full length; contains multiple point mutations
Entrez GeneSema3d (a.k.a. 4631426B19Rik)
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site Not1 (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that this vector was designed to generate a probe for in situ hybridization (ISH) and is functional for this purpose. The SEMA3D sequence is not full-length and may contain additional mutations when compared to the GenBank reference sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SEMA3D (mouse) was a gift from Jonathan Raper (Addgene plasmid # 29452 ; http://n2t.net/addgene:29452 ; RRID:Addgene_29452)