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SEMA3D (mouse)
(Plasmid #29452)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 29452 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SEMA3D
  • Alt name
    collapsin 2
  • Alt name
    semaphorin 3D
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    6000
  • Mutation
    insert is not full length; contains multiple point mutations
  • GenBank ID
    NM_028882
  • Entrez Gene
    Sema3d (a.k.a. 4631426B19Rik)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Please note that this vector was designed to generate a probe for in situ hybridization (ISH) and is functional for this purpose. The SEMA3D sequence is not full-length and may contain additional mutations when compared to the GenBank reference sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SEMA3D (mouse) was a gift from Jonathan Raper (Addgene plasmid # 29452 ; http://n2t.net/addgene:29452 ; RRID:Addgene_29452)