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pBS-mPlexinA2
(Plasmid #16423)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16423 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript II KS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    In Situ Template

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Plexin A2
  • Alt name
    Plexin 2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2200
  • Mutation
    None
  • GenBank ID
    D86949
  • Entrez Gene
    Plxna2 (a.k.a. 2810428A13Rik, AA589422, AW457381, OCT, PlexA2, Plxn2, mKIAA0463)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jonathan A. Raper, University of Pennsylvania
  • Terms and Licenses
  • Article Citing this Plasmid

Depositor Comments

This plasmid is an improved form of the mPlexin A2 in situ template plasmid originally described in Brown CB, Feiner L, Lu MM, Li J, Ma X, Webber AL, Jia L, Raper JA, Epstein JA. PlexinA2 and semaphorin signaling during cardiac neural crest development.
Development. 2001 128(16):3071-80. Anti-sense in situ probe is generated by linearizing with BamHI and transcribing with T3 RNA polymerase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-mPlexinA2 was a gift from Jonathan Raper (Addgene plasmid # 16423 ; http://n2t.net/addgene:16423 ; RRID:Addgene_16423)
  • For your References section:

    PlexinA2 and semaphorin signaling during cardiac neural crest development. Brown CB, Feiner L, Lu MM, Li J, Ma X, Webber AL, Jia L, Raper JA, Epstein JA. Development. 2001 Aug . 128(16):3071-80. PubMed 11688557