Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16423||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepBluescript II KS
- Backbone size w/o insert (bp) 3000
Vector typeIn Situ Template
Growth in Bacteria
Copy numberLow Copy
Gene/Insert namePlexin A2
Alt namePlexin 2
SpeciesM. musculus (mouse)
Insert Size (bp)2200
Entrez GenePlxna2 (a.k.a. 2810428A13Rik, AA589422, AW457381, OCT, PlexA2, Plxn2, mKIAA0463)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
This plasmid is an improved form of the mPlexin A2 in situ template plasmid originally described in Brown CB, Feiner L, Lu MM, Li J, Ma X, Webber AL, Jia L, Raper JA, Epstein JA. PlexinA2 and semaphorin signaling during cardiac neural crest development.
Development. 2001 128(16):3071-80. Anti-sense in situ probe is generated by linearizing with BamHI and transcribing with T3 RNA polymerase.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS-mPlexinA2 was a gift from Jonathan Raper (Addgene plasmid # 16423 ; http://n2t.net/addgene:16423 ; RRID:Addgene_16423)
For your References section:PlexinA2 and semaphorin signaling during cardiac neural crest development. Brown CB, Feiner L, Lu MM, Li J, Ma X, Webber AL, Jia L, Raper JA, Epstein JA. Development. 2001 Aug . 128(16):3071-80. PubMed 11688557