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(Plasmid #21934)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 21934 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    neuropilin 1
  • Alt name
    Nrp; NP-1; Npn1; NPN-1; C530029I03; Nrp1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    Nrp1 (a.k.a. C530029I03, NP-1, NPN-1, Npn1, Nrp)
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • Cherry (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site SacII (not destroyed)
  • 5′ sequencing primer CMV_fwd_primer
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Nrp1 cDNA was a gift from Dr. Andreas Püschel, Institut für Allgemeine Zoologie und Genetik Westfälische Wilhelms-Universität Münster
  • Articles Citing this Plasmid

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

AcGFP was substituted with Cherry from Roger Tsien's laboratory cloned between AgeI and NotI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCherry-mNrp1 was a gift from Guido Serini (Addgene plasmid # 21934 ; ; RRID:Addgene_21934)
  • For your References section:

    Neuropilin-1/GIPC1 signaling regulates alpha5beta1 integrin traffic and function in endothelial cells. Valdembri D, Caswell PT, Anderson KI, Schwarz JP, Koenig I, Astanina E, Caccavari F, Norman JC, Humphries MJ, Bussolino F, Serini G. PLoS Biol. 2009 Jan 27. 7(1):e25. 10.1371/journal.pbio.1000025 PubMed 19175293