|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||23945||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5005
Vector typeGateway Donor vector
Growth in Bacteria
Copy numberHigh Copy
Alt nameAXL receptor tyrosine kinase
SpeciesH. sapiens (human)
Insert Size (bp)2658
Entrez GeneAXL (a.k.a. ARK, JTK11, Tyro7, UFO)
- Cloning method Gateway Cloning
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13rev (Common Sequencing Primers)
The ORF has a Stop codon.
A set of 4 custom lentiviral vectors is available (pLX301 http://www.addgene.org/25895 , pLX302 http://www.addgene.org/25896 , pLX303 http://www.addgene.org/25897 , pLX304 http://www.addgene.org/25890 ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Addgene QC NGS analysis identifies a Q31P and Q764R mutation relative to NP_068713.2. As the Kinase ORFeome collection has been generated by high throughput methods using RNA from different tissues and sources, it is possible that this is a tissue specific isoform.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDONR223-AXL was a gift from William Hahn & David Root (Addgene plasmid # 23945 ; http://n2t.net/addgene:23945 ; RRID:Addgene_23945)
For your References section:COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA, Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM, Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA. Nature. 2010 Nov 24. ():. 10.1038/nature09627 PubMed 21107320