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PKC beta I WT
(Plasmid #16378)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 16378 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    PKC beta 1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
  • Entrez Gene
    Prkcb (a.k.a. Pkcb, Prkcb1)
  • Tag / Fusion Protein
    • HA (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

From user-provided sequence (see 'Reviews'), there are five amino acid differences from one of two NCBI reference sequences (AAA41868.1 and NP_001165776.1): 25A, 62F, 64K, 69C, and 294G. These "mutations" are also present in human, bovine, and xenopus sequences and are therefore not believed to affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PKC beta I WT was a gift from Bernard Weinstein (Addgene plasmid # 16378 ; ; RRID:Addgene_16378)
  • For your References section:

    Roles of specific isoforms of protein kinase C in the transcriptional control of cyclin D1 and related genes. Soh JW, Weinstein IB. J Biol Chem. 2003 Sep 5. 278(36):34709-16. 10.1074/jbc.M302016200 PubMed 12794082