|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17091||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepT7TS (based on pGEM4Z)
- Backbone size (bp) 3000
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
5' & 3' untranslated regions of Xenopus Beta-globin mRNA cloned into pGEM4Z. Insert size: 260 bp. Transcription: transcribe with T7 RNA polymerase. Cloning sites: Bgl II, EcoRV, SpeI
Xenbase has a nice description of this vector: http://www.xenbase.org/reagents/vectorAction.do?method=displayVectorSummary&vectorId=11536497&tabId=0
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pT7TS was a gift from Paul Krieg (Addgene plasmid # 17091 ; http://n2t.net/addgene:17091 ; RRID:Addgene_17091)