pT4/HB
(Plasmid #108352)

• Purpose: (Empty Backbone) Optimized Sleeping Beauty Transposon Vector with MCS
• Depositing Lab: Wolfgang Uckert
• Publication: Wang et al Nucleic Acids Res. 2017 Jan 9;45(1):311-326. doi: 10.1093/nar/gkw1164. Epub 2016 Dec 1.

Backbone

• Vector backbone: pBluescript
• Backbone manufacturer: Stratagene
• Backbone size (bp): 3457
• Modifications to backbone: Sleeping Beauty terminal inverted repeats inserted with MCS.
• Vector type: Transposon

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • pT4-HB.gb
• A portion of this plasmid was derived from a plasmid made by: Julian Clauß, Max-Delbrück-Center for Molecular Medicine, Berlin, Germany
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Sleeping Beauty transposon system consists of two components: the transposon vector and the transposase vector. The transposon vector (pT4/HB, Addgene #108352) contains binding sites flanking the GOI. And the transposase vector (pCMV(CAT)T7-SB100, Addgene #34879) encodes for the enzyme mediating excision and integration of the GOI.