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(Plasmid #117046)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117046 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 3457
  • Total vector size (bp) 4999
  • Modifications to backbone
    Sleeping Beauty terminal inverted repeats inserted which are engulfing a CMV-GFP transgene cassette.
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Insert Size (bp)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer GFP-FWD
  • 3′ sequencing primer GFP-REV
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Julian Clauß, Max-Delbrück-Center for Molecular Medicine, Berlin, Germany
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Sleeping Beauty transposon system consists of two components: the transposon vector and the transposase vector. The transposon vector (empty alternative: pT4/HB, Addgene #108352) contains binding sites flanking the GOI. And the transposase vector (pCMV(CAT)T7-SB100, Addgene #34879) encodes for the enzyme mediating excision and integration of the GOI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT4-CMV-GFP was a gift from Wolfgang Uckert (Addgene plasmid # 117046 ; ; RRID:Addgene_117046)