PurposeOptimized Sleeping Beauty Transposon Vector for GFP expression by a CMV promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||117046||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3457
- Total vector size (bp) 4999
Modifications to backboneSleeping Beauty terminal inverted repeats inserted which are engulfing a CMV-GFP transgene cassette.
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Insert Size (bp)720
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer GFP-FWD
- 3′ sequencing primer GFP-REV (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byJulian Clauß, Max-Delbrück-Center for Molecular Medicine, Berlin, Germany
Terms and Licenses
- Not Available to Industry
The Sleeping Beauty transposon system consists of two components: the transposon vector and the transposase vector. The transposon vector (empty alternative: pT4/HB, Addgene #108352) contains binding sites flanking the GOI. And the transposase vector (pCMV(CAT)T7-SB100, Addgene #34879) encodes for the enzyme mediating excision and integration of the GOI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pT4-CMV-GFP was a gift from Wolfgang Uckert (Addgene plasmid # 117046 ; http://n2t.net/addgene:117046 ; RRID:Addgene_117046)