166,461 results

1. SspBmicro-mCh-p60

   Plasmid

   #190168

   Purpose

   Expresses SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.

   Depositor

   Anna Akhmanova

   Article

   Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

   Insert

   SspBmicro-mCherry-p60
   

   Use

   Tags

   SspBmicro-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX303

   Plasmid

   #25897

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pyEvolvR (enCas9-PolI5M)

   Plasmid

   #158073

   Purpose

   Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   John Dueber

   Article

   Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

   Insert

   enCas9-PolI5M
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. dCas9-5xGCN4-P2A-BFP

   Plasmid

   #184438

   Purpose

   dCas9 fused with 5 copies of GCN4 epitope

   Depositor

   Rhys Allan

   Article

   Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

   Insert

   dCas9-5xGCN4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

   Plasmid

   #131461

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   Trp53-V5, H3F3A-EGFP-AU1

   Expression

   Mammalian

   Mutation

   Pdgfra D842V

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19_HDRT_TRAC_CD19-CAR

   Plasmid

   #183473

   Purpose

   Encodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC gene

   Depositor

   Dimitrios Wagner

   Article

   Kath et al Mol Ther Methods Clin Dev. 2022 Apr 12;2

   Inserts

   TRAC (TRAC Human)
   CD19-specific CAR transgene insert
   TRAC (TRAC Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV_hSynapsin_psychLight2 (AAV9)

   Viral Prep

   #163909-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV_hSynapsin_psychLight2 (#163909). In addition to the viral particles, you will also receive purified pAAV_hSynapsin_psychLight2 plasmid DNA. Synapsin-driven expression of the genetically encoded fluorescent sensor psychLight2 to detect behaviorally relevant serotonin release. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Lin Tian

   Article

   Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

   Promoter

   Syn

   Tags

   None

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

8. pFastBac-Dual-M2

   Plasmid

   #135584

   Purpose

   High eukaryotic dual protein expression shuttle vector

   Depositor

   Xiao-Wen Cheng

   Article

   Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

   Type

   Empty backbone

   Use

   Transfer vector

   Tags

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin of AcMNPV

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. VE-cad-eGFP Donor plasmid

   Plasmid

   #92309

   Purpose

   Knock eGFP into human VE-cad locus

   Depositor

   Sean Palecek

   Article

   Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

   Insert

   VEcad Homologous Recombination Arms (CDH5 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   about 30 a.a. truncated at end of protein

   Promoter

   Available Since

   Aug. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMBP-FnCas12a

    Plasmid

    #113432

    Purpose

    Expresses 10xHis-MBP fused to FnCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    FnCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRBad-Z-CspGFP

    Plasmid

    #40730

    Depositor

    Brian McNaughton

    Article

    Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

    Insert

    Leucine Zipper prime - C super positive Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBad

    Available Since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEBB-SBP-HA

    Plasmid

    #36100

    Depositor

    Ezra Burstein

    Type

    Empty backbone

    Use

    Tags

    HA and SBP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHCMV-SS-antiFLAGscFv-TM

    Plasmid

    #198303

    Purpose

    Displays anti-FLAG scFv from the surface of mammalian cells

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    pHCMV-SS-antiFLAGscFv-TM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. mc2 mNeon

    Plasmid

    #206218

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRS-LacI-GFP

    Plasmid

    #220198

    Purpose

    mini-Tn7 GFP expression vector - LacI/pLac promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    monomeric superfolder green fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    LacI/pLac

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLS-mP

    Plasmid

    #81225

    Purpose

    Lentivirus enhancer assay plasmid that contains a minimal promoter (mP) and a EGFP reporter gene.

    Depositor

    Nadav Ahituv

    Article

    Inoue et al Genome Res. 2016 Nov 9. pii: gr.212092.1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Derived from pGL4.23

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGIPz-mPD-L1

    Plasmid

    #121488

    Purpose

    Expression of mouse PD-L1 WT

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. TetOn-CLIMP63-mCherry-eGFP

    Plasmid

    #139871

    Purpose

    Dox-inducible lentiviral expression of human CLIMP63

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    CLIMP63 (CKAP4 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-eGFP

    Expression

    Mutation

    Promoter

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-OsTIR1(F74G)

    Plasmid

    #140730

    Purpose

    OsTIR1(F74G)-P2A-mAID-EGFP-NES

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(F74G)-P2A-mAID-EGFP-NES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

    Plasmid

    #179106

    Purpose

    pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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