168,499 results

1. pNCS BFP

   Plasmid

   #91757

   Purpose

   Bacterial expression of a fluorescent protein, Blue Fluorescent Protein (BFP)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

   Insert

   Blue Fluorescent Protein
   

   Tags

   Hexa-Histidine, Xpress epitope for detection with…

   Expression

   Bacterial

   Mutation

   GFP (Y66H)

   Promoter

   T7

   Available Since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Sybody Generation Toolbox

   Plasmid Kit

   #1000000160

   Purpose

   The Seeger Lab protocol to select synthetic nanobodies – called sybodies – against (membrane) protein targets. This collection contains all plasmids necessary for the sybody generation.

   Depositor

   Markus Seeger

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

3. Flag-ZDHHC5

   Plasmid

   #231654

   Purpose

   Expresses human ZDHHC5 protein

   Depositor

   Chunhong Ma

   Article

   Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

   Insert

   ZDHHC5 (ZDHHC5 Human)
   

   Tags

   3×Flag Tag

   Expression

   Mammalian

   Available Since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNCA

   Plasmid

   #17363

   Depositor

   Stephen Goff

   Article

   Colicelli et al J Mol Biol. 1988 Jan 5. 199(1):47-59.

   Insert

   MMLV proviral DNA
   

   Available Since

   March 1, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pLenti Lifeact-EGFP BlastR

   Plasmid

   #84383

   Purpose

   Lentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neurons

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   EGFP

   Promoter

   CMV immediate early

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF2932

   Plasmid

   #144408

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TFAP2A (TFAP2A Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBOBI C-FLAG PHGDH

   Plasmid

   #212995

   Purpose

   expresses human PHGDH

   Depositor

   Sheng-cai Lin

   Article

   Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

   Insert

   PHGDH (PHGDH Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV.CamKII.GCaMP6s.WPRE.SV40

   Plasmid

   #107790

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from the CamKII promoter

   Depositor

   James M. Wilson

   Has Service

   AAV9

   Insert

   GCaMP6s
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CamKII

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Hs.NRAS Q61R

   Plasmid

   #83179

   Purpose

   Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61R mutation

   Depositor

   Dominic Esposito

   Insert

   NRAS (NRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   Q61R

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6x-His_A1aY1_GFP

    Plasmid

    #158753

    Purpose

    Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

    Insert

    6xHistidine_A1aY1_GFP
    

    Tags

    6x-Histidine tag, GFP, T7 leader sequence, and Th…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. ITPR1_Halo_N_allele

    Plasmid

    #178161

    Purpose

    Donor vector for endogenous tagging of human ITPR1 at the N-terminus with halotag

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Halotag (ITPR1 Human)
    

    Use

    Donor vector

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-EF1a-Calr-KDEL-IRES-Puromycin

    Plasmid

    #134664

    Purpose

    Expresses EGFP-tagged endoplasmic reticulum marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    calreticulin (CALR Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 18-413

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC0043-PspCas13b-gRNA non target

    Plasmid

    #223698

    Purpose

    gRNA control for dPspCas13b-FTO

    Depositor

    Chuan He

    Article

    Wei et al Science. 2022 May 27;376(6596):968-973.

    Insert

    gRNA target sequence for luciferase control
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pfwB

    Plasmid

    #37329

    Depositor

    John Schiller

    Article

    Buck et al J Virol. 2005 Mar;79(5):2839-46.

    Insert

    GFP
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3 Flag Jnk3a1

    Plasmid

    #13758

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk3a1 (MAPK10 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-hSyn-Gluc-RMA-IRES-EGFP

    Plasmid

    #189629

    Purpose

    Expresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Inserts

    Gaussia luciferase fused to Fc
    IRES-EGFP
    

    Use

    AAV and Luciferase

    Tags

    Gluc and IgG1 Fc

    Expression

    Mammalian

    Promoter

    IRES and hSyn

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. p426_Cas9_gRNA-ARS208a

    Plasmid

    #87383

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS208a sequence GTCCGCTAAACAAAAGATCT in yeast chromosome 2.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS208a
    

    Use

    CRISPR

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST

    Plasmid

    #49771

    Purpose

    Level 1 hCas9 module

    Depositor

    Sophien Kamoun

    Article

    Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

    Insert

    35Sp::hCas9
    

    Use

    CRISPR

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. FR_HNF4A8

    Plasmid

    #31114

    Depositor

    Gerhart Ryffel

    Article

    Erdmann et al Biol Chem. 2007 Jan . 388(1):91-106.

    Insert

    HNF4A (HNF4A Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    splice variant 8

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.1-FLAG-hSF3B1-K700E

    Plasmid

    #82577

    Purpose

    Expresses a codon-optimized ORF of human SF3B1 ( K700E mutant)

    Depositor

    Manoj Pillai

    Article

    Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

    Insert

    human SF3B1-K700E (SF3B1 Human)
    

    Tags

    FLAG peptide

    Expression

    Mammalian

    Mutation

    K700E

    Promoter

    CMV

    Available Since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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