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An optimized retroviral toolbox for overexpression and genetic perturbation of primary lymphocytes.
van der Donk LEH, van der Spek J, van Duivenvoorde T, Ten Brink MS, Geijtenbeek TBH, Kuijl CP, van Heijst JWJ, Ates LS
Biol Open. 2022 Feb 15;11(2):bio059032. doi: 10.1242/bio.059032. Epub 2022 Mar 1. PubMed Article

Plasmids from Article

ID Plasmid Purpose
163324pMX-hPGK-CD90.2-Cd247A_miRRetroviral vector for knockdown of murine CD247A (TCR zeta) and expression of a CD90.2 surface marker under control of the human PGK promoter.
163325pMX-hFTH1-CD90.2-Cd247A_miRRetroviral vector for knockdown of murine CD247A (TCR zeta) and expression of a CD90.2 surface marker under control of the human FTH1 promoter.
163326pMX-CAG-CD90.2-Cd247A_miRRetroviral vector for knockdown of murine CD247A (TCR zeta) and expression of a CD90.2 surface marker under control of the CAG promoter.
163327pMX-mPGK-CD90.2-Cd247A_miRRetroviral vector for knockdown of murine CD247A (TCR zeta) and expression of a CD90.2 surface marker under control of the murine PGK promoter.
163328pMY-CD90.2-Cd247A_miRRetroviral vector for knockdown of murine CD247A (TCR zeta) and expression of a CD90.2 surface marker under control of the LTR promoter.
163329pMX-hPGK-CD90.2-Rluc_miRRetroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the human PGK promoter.
163330pMX-hFTH1-CD90.2-Rluc_miRRetroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the human FTH1 promoter.
163331pMX-CAG-CD90.2-Rluc_miRRetroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the CAG promoter.
163332pMX-mPGK-CD90.2-Rluc_miRRetroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the murine PGK promoter.
163333pMY-CD90.2-Rluc_miRRetroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the LTR promoter.
163334pMX-hPGK-CD3-P2A-CD90.2Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the hPGK promoter
163335pMX-hFTH1-CD3-P2A-CD90.2Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the hFTH1 promoter
163336pMX-CAG-CD3-P2A-CD90.2Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the CAG promoter
163337pMX-mPGK-CD3-P2A-CD90.2Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the mPGK promoter
163338pMY-CD3-P2A-CD90.2Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the LTR promoter
163339pMY-Ly6G-Rluc_miRRetroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker
163340pMY-Ly6G-P2A-PuroR-HA-Rluc_miRRetroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker and an HA-tagged puromycin selection marker
163341pMY-CD90.2-P2A-BlastDYK-Rluc_miRRetroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker and a DYK-tagged Blasticidin selection marker
163342pMY-mCherry-P2A-PuroR-HARetroviral vector to express cytosolic mCherry and an HA-tagged puromycin selection marker
163343pMY-eGFP-P2A-PuroR-HARetroviral vector to express cytosolic GFP and an HA-tagged puromycin selection marker
163344pMY-mTurquoise2-P2A-PuroR-HARetroviral vector to express cytosolic mTurquoise2 and an HA-tagged puromycin selection marker
163345pMY-mVenus-P2A-PuroR-HARetroviral vector to express cytosolic mVenus and an HA-tagged puromycin selection marker
163346pMY-Lyz2-GSG-mCherryRetroviral vector to express C-terminally mCherry-tagged murine Lyz2
163347pMY-Lyz2-GSG-mTurquoiseRetroviral vector to express C-terminally mTurquoise2-tagged murine Lyz2
163348pMY-Lyz2-GSG-mVenusRetroviral vector to express C-terminally mVenus-tagged murine Lyz2
163349pMY-CD90.2-P2A-BlastDYKRetroviral vector to express the surface marker CD90.2 and a DYK-tagged blasticidin resistance marker
163350pMY-MCS-GSG-eGFPRetroviral backbone for in-frame cloning of a C-terminally GFP labeled gene of interest.
163351pMY-empty-MCSThe empty pMY backbone with multiple cloning site
163352pMY-MCS-P2A-PuroR-HARetroviral backbone for in-frame cloning of a gene of interest and a P2A separated Puromycin selection marker
163353pMY-MCS-P2A-Ly6GRetroviral backbone for in-frame cloning of a gene of interest and a P2A separated Ly6G surface marker
163354pMY-MCS-P2A-CD90.1Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated CD90.1 surface marker
163355pMY-MCS-P2A-CD90.2Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated CD90.2 surface marker
163356pMY-MCS-P2A-eGFPRetroviral backbone for in-frame cloning of a gene of interest and a P2A separated GFP marker
163357pMY-MCS-P2A-BlastR-DYKRetroviral backbone for in-frame cloning of a gene of interest and a P2A separated Blasticidin selection marker
163358pLenti-EF1a-Ly6G-miR30Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker.
163359pLenti-EF1a-Ly6G-P2A-PuroR-HA-miR30Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a Ly6G surface marker and a puromycin selection marker.
163360pLenti-EF1a-CD90.2-Rluc_miRLentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a CD90.2 surface marker.
163361pMY-IRES-eGFPRetroviral backbone for in-frame cloning of a gene of interest and an IRES separated GFP marker
163362pLenti-EFS-MCS-WPREThe empty pLenti backbone with multiple cloning site that allows shuttling between pMY and pMX vectors

Antibodies from Article