Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pMX-hFTH1-CD90.2-Cd247A_miR
(Plasmid #163325)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 163325 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD90.2
  • Alt name
    Thy1b
  • gRNA/shRNA sequence
    CCTCTTCATCTACGGAGTCAT
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Thy1 (a.k.a. CD90, T25, Thy-1, Thy-1.2, Thy1.1, Thy1.2)
  • Promoter hFTH1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMX-hFTH1-CD90.2-Cd247A_miR was a gift from Louis Ates & Teunis Geijtenbeek (Addgene plasmid # 163325 ; http://n2t.net/addgene:163325 ; RRID:Addgene_163325)
  • For your References section:

    An optimized retroviral toolbox for overexpression and genetic perturbation of primary lymphocytes. Van der Donk L, Van der Spek J, Van Duivenvoorde T, Brink M, Geijtenbeek T, Kuijl C, Van Heijst J, Ates L. bioRxiv 10.1101/2021.01.08.425881