|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||163325||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Retroviral, RNAi
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Entrez GeneThy1 (a.k.a. CD90, T25, Thy-1, Thy-1.2, Thy1.1, Thy1.2)
- Promoter hFTH1
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMX-hFTH1-CD90.2-Cd247A_miR was a gift from Louis Ates & Teunis Geijtenbeek (Addgene plasmid # 163325 ; http://n2t.net/addgene:163325 ; RRID:Addgene_163325)
For your References section:An optimized retroviral toolbox for overexpression and genetic perturbation of primary lymphocytes. Van der Donk L, Van der Spek J, Van Duivenvoorde T, Brink M, Geijtenbeek T, Kuijl C, Van Heijst J, Ates L. bioRxiv 10.1101/2021.01.08.425881