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A reference library for assigning protein subcellular localizations by image-based machine learning.
Schormann W, Hariharan S, Andrews DW
J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 10.1083/jcb.201904090.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
133025pLVX-EF1a-EGFP-TA MAO-IRES-PuromycinExpresses EGFP-tagged tail anchor sequence of monoamine oxidase A
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133026pLVX-EF1a-EGFP-TA Bik-IRES-PuromycinExpresses EGFP-tagged tail anchor sequence of Bik
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133027pLVX-EF1a-EGFP-RAB7A-IRES-PuromycinExpresses EGFP-tagged late endosome marker
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133028pLVX-EF1a-EGFP-TA Golgin84-IRES-PuromycinExpresses EGFP-tagged Golgi apparatus marker
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134661pLVX-EF1a-EGFP-VAMP2-IRES-PuromycinExpresses EGFP-tagged synaptic vesicle protein
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134662pLVX-EF1a-EGFP-VAMP5-IRES-PuromycinExpresses EGFP-tagged plasma membrane marker
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134664pLVX-EF1a-Calr-KDEL-IRES-PuromycinExpresses EGFP-tagged endoplasmic reticulum marker
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134665pLVX-EF1a-PTS1-IRES-PuromycinExpresses EGFP-tagged peroxisome marker
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134666pLVX-EF1a-Neuromodulin-IRES-PuromycinExpresses EGFP-tagged plasma membrane marker
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134667pLVX-EF1a-PTDSS1-IRES-PuromycinExpresses EGFP-tagged Mitochondria-associated ER membrane marker
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134857pLVX-EF1a-EGFP-RAB3C-IRES-PuromycinExpresses EGFP-tagged synaptic vesicle marker
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134858pLVX-EF1a-EGFP-RAB5A-IRES-PuromycinExpresses EGFP-tagged early endosome marker
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134859pLVX-EF1a-EGFP-ERGIC53-IRES-PuromycinExpresses EGFP-tagged ERGIC marker
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134860pLVX-EF1a-EGFP-RAB11B-IRES-PuromycinExpresses EGFP-tagged recycling endosome marker
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134861pLVX-EF1a-CCO-IRES-PuromycinExpresses EGFP-tagged inner mitochondrial membrane marker
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134862pLVX-EF1a-GalT-IRES-PuromycinExpresses EGFP-tagged Golgi marker
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134863pLVX-EF1a-EGFP-RAMP4-IRES-PuromycinExpresses EGFP-tagged endoplasmic reticulum marker
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134864pLVX-EF1a-EGFP-Emerin-IRES-HygromycinExpresses EGFP-tagged nuclear envelope marker
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134865pLVX-EF1a-EGFP-Membrin-IRES-PuromycinExpresses EGFP-tagged Golgi apparatus marker
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134866pLVX-EF1a-delta-TMD-VAMP1-IRES-HygromycinExpresses EGFP-tagged mutant of VAMP1
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134867pLVX-EF1a-GFP-LaminA-IRES-HygromycinExpresses EGFP-tagged nuclear envelope marker
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134868pLVX-EF1a-LAMP-1-mGFP-IRES-PuromycinExpresses EGFP-tagged lysosomal marker
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134869pLVX-EF1a-EGFP-Cybt5-IRES-PuromycinExpresses EGFP-tagged endoplasmic reticulum marker
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Antibodies from Article