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pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin
(Plasmid #133027)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 133027 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLVX-EF1alpha-IRES-Puromycin
  • Backbone size w/o insert (bp) 8825
  • Total vector size (bp) 10182
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAB7A
  • Alt name
    Ras-related protein Rab-7a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    624
  • Entrez Gene
    RAB7A (a.k.a. CMT2B, PRO2706, RAB7)
  • Promoter Human elongation factor 1 alpha
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer aag cgc gat cac atg gtc ctg
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin was a gift from David Andrews (Addgene plasmid # 133027 ; http://n2t.net/addgene:133027 ; RRID:Addgene_133027)
  • For your References section:

    A reference library for assigning protein subcellular localizations by image-based machine learning. Schormann W, Hariharan S, Andrews DW. J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 10.1083/jcb.201904090. 10.1083/jcb.201904090 PubMed 31968357