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EGFP-LC3

Plasmid

#11546

Depositor

Karla Kirkegaard

Article

Jackson et al PLoS Biol. 2005 May . 3(5):e156.

Insert

LC3B (MAP1LC3B Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pBT281

Plasmid

#122611

Purpose

express evoAPOBEC1-BE4max in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoAPOBEC1-BE4max

Expression

Mammalian

Mutation

E4K H109N H122L D124N R154H A165S P201S F205S

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBT281

Plasmid

#122611

Purpose

express evoAPOBEC1-BE4max in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoAPOBEC1-BE4max

Expression

Mammalian

Mutation

E4K H109N H122L D124N R154H A165S P201S F205S

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Puro-EF-1α>{H2B-RFP}

Plasmid

#201121

Purpose

RFP expression in cells under EF-1α promoter; cytoplasmic and histone-bound, nuclear

Depositor

Ori Bar-Nur

Article

Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

Insert

H2B-RFP

Use

Lentiviral

Mutation

No

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Puro-EF-1α>{H2B-RFP}

Plasmid

#201121

Purpose

RFP expression in cells under EF-1α promoter; cytoplasmic and histone-bound, nuclear

Depositor

Ori Bar-Nur

Article

Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

Insert

H2B-RFP

Use

Lentiviral

Mutation

No

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pJS101

Plasmid

#118280

Purpose

Expresses GFPmut2 with low cell-to-cell variation on a low-copy plasmid and tetracycline induction

Depositor

Zach Hensel

Article

Silva et al mSphere. 2019 May 29;4(3). pii: 4/3/e003

Insert

GFPmut2

Expression

Bacterial

Promoter

pLtetO-1

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pJS101

Plasmid

#118280

Purpose

Expresses GFPmut2 with low cell-to-cell variation on a low-copy plasmid and tetracycline induction

Depositor

Zach Hensel

Article

Silva et al mSphere. 2019 May 29;4(3). pii: 4/3/e003

Insert

GFPmut2

Expression

Bacterial

Promoter

pLtetO-1

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-SpCas9-HF1 (without sgRNA)

Plasmid

#92102

Purpose

Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A

Promoter

Cbh

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-SpCas9-HF1 (without sgRNA)

Plasmid

#92102

Purpose

Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A

Promoter

Cbh

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_PA-Cre

Plasmid

#122960

Purpose

Photoactivatable Cre recombinase based on split Cre fragments and Magnet system (pMag and nMag)

Depositor

Moritoshi Sato

Article

Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

Insert

pcDNA3.1_PA-Cre

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_PA-Cre

Plasmid

#122960

Purpose

Photoactivatable Cre recombinase based on split Cre fragments and Magnet system (pMag and nMag)

Depositor

Moritoshi Sato

Article

Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

Insert

pcDNA3.1_PA-Cre

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG LAP2

Plasmid

#15738

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LAP2 (CEBPB Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG LAP2

Plasmid

#15738

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LAP2 (CEBPB Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

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FLAG-SFPQ

Plasmid

#166960

Purpose

Expresses FLAG-tagged SFPQ protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ (SFPQ Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-SFPQ

Plasmid

#166960

Purpose

Expresses FLAG-tagged SFPQ protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ (SFPQ Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-mAirn-R-loop

Plasmid

#134900

Purpose

Generation and purification of R-loops for in vitro biochemistry

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

mAirn promoter (Airn Mouse)

Use

Synthetic Biology

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-mAirn-R-loop

Plasmid

#134900

Purpose

Generation and purification of R-loops for in vitro biochemistry

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

mAirn promoter (Airn Mouse)

Use

Synthetic Biology

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-GG-PUF

Plasmid

#52137

Purpose

Receiving vector for PUM-HD assembly and expression in E.coli

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Type

Empty backbone

Tags

PUM-HD flanking region 1' and PUM-HD flankin…

Expression

Bacterial

Promoter

T7

Available Since

May 5, 2014

Availability

Academic Institutions and Nonprofits only

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pET28-GG-PUF

Plasmid

#52137

Purpose

Receiving vector for PUM-HD assembly and expression in E.coli

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Type

Empty backbone

Tags

PUM-HD flanking region 1' and PUM-HD flankin…

Expression

Bacterial

Promoter

T7

Available Since

May 5, 2014

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6s-CAAX

Plasmid

#52228

Purpose

Plasma membrane targeted GCaMP6s, calcium sensor

Depositor

Tobias Meyer

Article

Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

Insert

GCaMP6s-CAAX

Tags

6xHis and CAAX

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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