167,318 results

1. pRK2-AraE

   Plasmid

   #110141

   Purpose

   Constitutive promoter expressing araE on RK2 origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   araE
   

   Expression

   Bacterial

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW57.1_AOX-FLAG

   Plasmid

   #177984

   Purpose

   All-in-one dox-inducible expression of Alternative Oxidase

   Depositor

   David Sabatini, Jessica Spinelli

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   AOX
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Codon optimized cDNA for expression in human cells

   Promoter

   TRE

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. M43: pHR-SFFVp-iLID::EGFP::FTH1

   Plasmid

   #122149

   Purpose

   Self-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   FTH1 (FTH1 Human)
   

   Use

   Lentiviral

   Tags

   NLS-iLID-GFP

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. NES-EGFP-PH-ARNO2G-I303Ex2

   Plasmid

   #116868

   Purpose

   PIP3 biosensor

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   CYTH2 (CYTH2 Human)
   

   Tags

   EGFP and X.leavis map2k1.L(32-44)

   Expression

   Mammalian

   Mutation

   amind acids 252-399, isoleucine 303 changed to gl…

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Humagne Set C

   Pooled Library

   #172650

   Purpose

   Set C contains best-ranked guides 1 and 4.

   Depositor

   David Root, John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Expression

   Mammalian

   Available Since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

6. CMV-G-GECO1.0

   Plasmid

   #32447

   Purpose

   Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.0)

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   G-GECO1.0
   

   Expression

   Mammalian

   Mutation

   GCaMP3 K119I/L173Q/S404G/E430V

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK198 (GAL-OsTIR1)

   Plasmid

   #140655

   Purpose

   GAL-OsTIR1(WT)

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   OsTIR1(WT)
   

   Expression

   Yeast

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. TALEN-CCR5L18-CtermQ3

   Plasmid

   #51440

   Purpose

   Expresses TALEN targeting left CCR5A site (L18) with Q3 C-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Left (L18) Q3 C-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 EL variant

   Expression

   Mammalian

   Mutation

   K788Q, R792Q and R801Q

   Promoter

   CMV

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. TALEN-CCR5R18-CtermQ3

   Plasmid

   #51441

   Purpose

   Expresses TALEN targeting right CCR5A site (R18) with Q3 C-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Right (R18) Q3 C-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 KK variant

   Expression

   Mammalian

   Mutation

   K788Q, R792Q and R801Q

   Promoter

   CMV

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. c-Flag pcDNA3

    Plasmid

    #20011

    Purpose

    This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

    Depositor

    Stephen Smale

    Article

    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Ef1a-Con/Fon-sRGECO (AAV8)

    Viral Prep

    #137126-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Fon-sRGECO (#137126). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-sRGECO plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of cytosolic sRGECO calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    EF1a

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

12. PCMV10-RBPJ

    Plasmid

    #84601

    Purpose

    Mammalian expression of FLAG tagged human RBPJ

    Depositor

    Yanan Yang

    Article

    Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

    Insert

    RBPJ (RBPJ Human)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. LAMP1-APEX2

    Plasmid

    #215555

    Purpose

    Stable, inducible expression in lysosome

    Depositor

    Nevan Krogan

    Article

    Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

    Insert

    LAMP1-EGFP-Flag tag-APEX2-NES
    

    Use

    Lentiviral

    Available Since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry KNL1 wt hardened

    Plasmid

    #45224

    Depositor

    Michael Lampson, Iain Cheeseman

    Article

    Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

    Insert

    mCherry KNL1 resistant to KNL1 siRNA (KNL1 Human)
    

    Tags

    TEV S15

    Expression

    Mammalian

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pTRE3G-Bi-gePSI-pCamk2a-AcGFP

    Plasmid

    #133732

    Purpose

    Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a Camk2a promoter.

    Depositor

    Erin Schuman

    Article

    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Inserts

    gePSI-β-chain
    gePSI-α-chain
    AcGFP1
    

    Use

    AAV

    Tags

    murine ornithine decarboxylase - degron (ODC36)

    Expression

    Mammalian

    Promoter

    pCamk2a and pTRE3G-Bi

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS415-GPD

    Plasmid

    #183940

    Purpose

    Control vector that has a GPD promoter with no insert

    Depositor

    Michael Downey

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-HAPLN1

    Plasmid

    #200777

    Purpose

    Expresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoter

    Depositor

    Alexander Dityatev

    Article

    Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

    Insert

    Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
    

    Use

    AAV

    Tags

    cfGFP

    Promoter

    Synapsin

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHCMV_CHIKV_E1E2

    Plasmid

    #207261

    Purpose

    Mammalian expression of Chikungunya virus E1E2 protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    CHIKV_E1E2 (CHIKVgp2 Chikungunya virus)
    

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-N1-FLAG

    Plasmid

    #60360

    Purpose

    Plasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLIX403-hNOS2

    Plasmid

    #110800

    Purpose

    tet-inducible NOS2 expression

    Depositor

    Edward Morgan

    Article

    Lee et al Free Radic Biol Med. 2017 Jul;108:478-48

    Insert

    NOS2 (NOS2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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