175,378 results
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Plasmid#200307PurposeBacterial expression plasmids for production of C247A mutant of asparaginyl endopeptidase 1 from O. affinis (OaAEP1_C247A)DepositorInsertAsparaginyl endopeptidase from Oldenlandia affinis
TagsSmt3ExpressionBacterialMutationCysteine 247 to AlaninePromoterT7Available SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Rheb (Q64L)-pcw107
Plasmid#64607Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT5_TRE-ARID1A-FLAG_PGK-H2B-RFP
Plasmid#203965PurposeDox inducible FLAG tag ARID1a in a sleeping beauty transposon constructDepositorInsertARID1A (Arid1a Mouse)
UseSleeping beauty transposonTagsFLAGExpressionMammalianPromoterTREAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet1 CD
Plasmid#70129PurposeExpress flag-tagged mouse Tet1 catalytic domain.DepositorInsertTen-Eleven Translocation 1 (Tet1 Mouse)
TagsflagExpressionMammalianMutationThe plasmid expresses TET1 catalytic domain.Available SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST (AAV9)
Viral Prep#109048-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST (#109048). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST plasmid DNA. Cre-dependent expression of the anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites, translated from an internal ribosomal entry site (IRES) following mRuby3 expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsmRuby3Available SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Alpha-Actinin-19
Plasmid#54975PurposeLocalization: Alpha Actinin, Excitation: 587, Emission: 610DepositorAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
PGK-H2BmCherry
Plasmid#21217DepositorAvailable SinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFP barcode-5-SV40 polyA
Plasmid#190869PurposeFor barcoded retrograde labelingDepositorInsertEGFP-barcode5
UseAAVMutationWTAvailable SinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mouse sgRNA library Brie in lentiCRISPRv2
Pooled Library#73632PurposeMouse sgRNA library in backbone lentiCRISPRv2 containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controlsDepositorExpressionMammalianUseCRISPR and LentiviralAvailable SinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Hygro-GFP
Plasmid#61215PurposeRetrovirus expressing GFPDepositorInsertGFP
UseRetroviralAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgOpti
Plasmid#85681PurposeLentiviral vector to express sgRNAs. The cloning site is BsmBI.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W
Plasmid#67986PurposeCas9 activity reporter with mCherry and BFPDepositorInsertsU6gRNA cassette, PGKmCherryABFP cassette, WPRE
Guide RNA targeting modified BFP
UseCRISPR and LentiviralMutationDeleted BbsI site within WPRE, modified BFPAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGE_En03
Plasmid#71535PurposeUsed to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRAvailable SinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
p65-mNeonGreen
Plasmid#127172PurposeFluorescently tagged p65 reporterDepositorAvailable SinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral-TOP-dGFP-reporter
Plasmid#14715PurposeBeta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).DepositorAvailable SinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
EF1a mCherry
Plasmid#129431Purposeexpression of mCherry from EF1a promoterDepositorInsertmCherry
UseLentiviralAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-α-cateninWT
Plasmid#229709PurposeTransient expression of GFP-alpha-cateninWT in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
TagsEGFPExpressionMammalianPromoterCMV promoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lyso-GA
Plasmid#209874PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the lysosomal membrane with human LAMP1DepositorInsertddGFP A
TagsLAMP1ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMIIS1011
Plasmid#238212PurposeExpresses ParTasR with an N-terminal HA-NLS tag under CMV promoter in human cells.DepositorInsertParTasR
TagsHA-NLSExpressionMammalianAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ccdB-V5
Plasmid#214985PurposeGateway compatible empty vector (transient mammalian expression) with a C-terminal V5 tagDepositorTypeEmpty backboneTagsV5ExpressionMammalianAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only